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169,913 results

Showing: 11971 - 12000 of 169913 results

  2. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid
    #181745
    Purpose
    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    human/zebrafish codon optimized SpCas9
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181745-plasmid-map-sequence-id-354676

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  7. NOVA1 eGFP

    Plasmid
    #61275
    Purpose
    GFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    NOVA1 (NOVA1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61275-plasmid-map-sequence-id-99586

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  12. pOPINP

    Plasmid
    #41139
    Depositor
    Ray Owens
    Type
    Empty backbone
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    March 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    41139-plasmid-map-sequence-id-54733

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  16. TFORF0107

    Plasmid
    #143010
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TRERF1 (TRERF1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143010-plasmid-map-sequence-id-398675

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  20. p663-UBC-V5-3xHA-miniTurbo-3XNLS-STOP_IDG-K

    Plasmid
    #158160
    Purpose
    Gateway destination clone of miniTurbo-3XNLS (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-3xHA-miniTurbo-3XNLS
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    158160-plasmid-map-sequence-id-309971

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  21. TFORF0169

    Plasmid
    #141540
    Purpose
    Lentiviral vector for overexpressing the SIX3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SIX3 (SIX3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141540-plasmid-map-sequence-id-276756

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  23. MDKA-c012

    Plasmid
    #205113
    Purpose
    MDK protein expression
    Depositor
    Paul Brennan
    Insert
    MDK (MDK Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    205113-plasmid-map-sequence-id-410384

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  25. pLV-REPORT(PGK)

    Plasmid
    #172328
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172328-plasmid-map-sequence-id-354688

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  26. pRDA_221

    Plasmid
    #169142
    Purpose
    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.
    Depositor
    John Doench, David Root
    Insert
    EGFP , EGFP sgRNA
    Use
    CRISPR and Lentiviral
    Available Since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    169142-plasmid-map-sequence-id-334957

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  28. TFORF1450

    Plasmid
    #143810
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DLX6 (DLX6 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143810-plasmid-map-sequence-id-396252

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Showing: 11971 - 12000 of 169913 results