163,445 results

1. TrpC5 scFv [N67/15]

   Plasmid

   #190564

   Purpose

   Mammalian Expression of TrpC5 scFV. Derived from hybridoma N67/15.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   TrpC5 (Homo sapiens) recombinant scFV (TRPC5 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-FGFR3__TACC3

   Plasmid

   #116818

   Purpose

   Lentiviral expression of FGFR3__TACC3 fusion

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   FGFR3__TACC3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. beta-arrestin1 mYFP

   Plasmid

   #36916

   Depositor

   Robert Lefkowitz

   Article

   Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

   Insert

   beta-arrestin1 (Arrb1 Rat)
   

   Use

   Tags

   mYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. mRuby2-pBAD

   Plasmid

   #54771

   Purpose

   Localization: Protein Expression Vector , Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Tags

   6xHis and mRuby2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pSLIK-Hygro

   Plasmid

   #25737

   Purpose

   3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1+-Tornado-split-nLuc

   Plasmid

   #212612

   Purpose

   For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   Tornado-CVB3-split-nLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   C373T mutation in CVB3

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. HP35 tension sensor

   Plasmid

   #101250

   Purpose

   Expresses the HP35-based tension sensor module. The biosensor allows force measurements at 6-8 pN.

   Depositor

   Carsten Grashoff

   Article

   Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

   Insert

   YPet(short)-HP35-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TPC1-G-GECO1.2

   Plasmid

   #207143

   Purpose

   Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   TPCN1 (TPCN1 Human)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19/PLIN2 GFP-P2A-BFP

   Plasmid

   #202195

   Purpose

   knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   Cloning vector

   Tags

   GFP-P2A-BFP knock-in

   Expression

   Mutation

   Promoter

   lac

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW-HA-hRb-delta-CDK-puro

    Plasmid

    #212673

    Purpose

    Express tagged hRb delta-CDK phosphosite mutant

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    Rb (RB1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    All 15 CDK phospho-sites are mutated to alanines

    Promoter

    TRE

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBTK503

    Plasmid

    #110616

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. GLASTp-DsRed2

    Plasmid

    #17706

    Depositor

    Nicholas Gaiano

    Article

    Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

    Insert

    GLASTp (SLC1A3 Human)
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF0056

    Plasmid

    #142529

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MAF (MAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. SARS-CoV-2_N_FL

    Plasmid

    #157867

    Purpose

    express MBP tagged SARS-CoV-2 Nucleocapsid protein

    Depositor

    Nicolas Fawzi

    Article

    Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

    Insert

    N (N SARS-CoV-2)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109

    Plasmid

    #111730

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q109 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. LentiGuide-10X-CS1-3'-puro

    Plasmid

    #212011

    Purpose

    Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)

    Depositor

    Danwei Huangfu

    Insert

    gRNA scaffold
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

    Plasmid

    #209078

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    3K mutant: E35K, E46K, E61K

    Promoter

    TRE4G

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC25A11

    Plasmid

    #132026

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A11 (SLC25A11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. GPD-roGFP

    Plasmid

    #49436

    Purpose

    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells

    Depositor

    Paul Schumacker

    Article

    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

    Insert

    GPD-roGFP2
    

    Use

    Adenoviral

    Tags

    Glycerol phosphate dehydrogenase, 5' to roGF…

    Expression

    Mammalian

    Mutation

    C48S, Q80R, S147C, and Q204C in Clontech's G…

    Promoter

    CMV

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3xFLAG-NanoLuc

    Plasmid

    #189975

    Purpose

    Part 3-4-5 plasmid to introduce 3xFLAG-NanoLuc into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Cloning

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. lentiCRISPRv2_RB1#1

    Plasmid

    #174150

    Purpose

    Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    RB1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. R4pGWB601_UBQ10p-Turbo-NES-YFP

    Plasmid

    #127366

    Purpose

    Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBMN mEGFP-NDP52

    Plasmid

    #188785

    Purpose

    For mammalian expression of NDP52 tagged with mEGFP

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3/HF-CDC50A

    Plasmid

    #209223

    Purpose

    Mammalian expression of CDC50A

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    CDC50A (TMEM30A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EK0498 SFFV-mCherry-SGc(ACTB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191182

    Purpose

    Expression of EACTB sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:ACTB.s1:EGFP-3xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mEGFP-Keratin-17

    Plasmid

    #56465

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. Plasmid L5 attB::Pleft* mScarlet

    Plasmid

    #169410

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mScarlet proper
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TPC2-G-GECO1.2

    Plasmid

    #207141

    Purpose

    Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPC2N2 (TPCN2 Human)
    

    Use

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PG1424PGA

    Plasmid

    #74652

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PG1424PGA
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pFLAG-SURF4

    Plasmid

    #170680

    Purpose

    Expresses FLAG-tagged SURF4 in mammalian cells.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    SURF4
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge