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pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

Plasmid

#69224

Purpose

Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS and Zif268

Expression

Mammalian

Mutation

Arginine 1335 is changed to Lysine

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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PX458_MBD1_iso1_2

Plasmid

#86304

Purpose

Encodes gRNA for 3' target of human MBD1_iso1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MBD1_iso1 (MBD1 Human)

Use

CRISPR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL11057

Plasmid

#68253

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promote:TaU6+sgRNA HvPM19_1

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 humanN-Flag SadBS

Plasmid

#66881

Purpose

Mammalian expression vector for expressing N-terminally Flag-tagged short isoform of SadB

Depositor

Maria Alvarado-Kristensson

Article

Eklund et al J Biol Chem. 2014 Aug 1;289(31):21360-73

Insert

SadB (BRSK1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pYZ_NAND2_L17_S19_S11

Plasmid

#132730

Purpose

T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND2_L17_S19_S11

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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CMYA5/pACT2

Plasmid

#97208

Purpose

CMYA5 (aa 4039 – 4069) in a GAL4 AD vector, used for yeast-two hybrid

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Expression

Yeast

Available Since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX 4T1 hBad (1-146)

Plasmid

#35569

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

bad (BAD Human)

Tags

Flag

Expression

Bacterial

Mutation

residues 1-146

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only
Opie2-NeoR[traF]+Opie2-PuroR[dsxM]

Plasmid

#131618

Purpose

Expresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Inserts

PuroR[dsxM]
NeoR[traF]

Expression

Insect

Promoter

Opie2

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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p224 pCMV-RFP/CREM

Plasmid

#8401

Purpose

Floxed RFP inserted within the coding sequence of cre recombinase.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

RFP/CREM

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre, LOX 511 sites surrounding RFP withi…

Promoter

CMV

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
RBMS3_2xRRM_pGEX

Plasmid

#135142

Purpose

Recombinant expression of RBMS3 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBMS3 (RBMS3 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

July 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSP64R1 SHP2 WT

Plasmid

#8325

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Xenopus expression

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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PX458_GATAD2A_2

Plasmid

#86334

Purpose

Encodes gRNA for 3' target of human GATAD2A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against GATAD2A (GATAD2A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_GATAD2A_1

Plasmid

#86333

Purpose

Encodes gRNA for 3' target of human GATAD2A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against GATAD2A (GATAD2A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-GW1D1a(aa254-503)

Plasmid

#21520

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa254-503

Available Since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only
RBM24_RRM_pGEX

Plasmid

#135148

Purpose

Recombinant expression of RBM24 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM24 (RBM24 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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RAB14 3'UTR D1D2

Plasmid

#61492

Purpose

luciferase reporter containing RAB14 3' UTR with both predicted MiR144 binding site deleted

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 3' UTR (RAB14 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains position 837-1637 (numbering relative to…

Promoter

PGK

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pIK137

Plasmid

#65632

Purpose

Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment

Expression

Worm

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pTJK512

Plasmid

#121469

Purpose

CEN LEU2 CNB1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Promoter

Endogenous genomic promoter region

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX2T merlin 340-590

Plasmid

#11633

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal half of merlin isoform 2.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pSP64R1 SHP2 D61A

Plasmid

#8326

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Xenopus expression

Mutation

D61A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
mPA-GFP-Paxillin-22

Plasmid

#57153

Purpose

Localization: Focal Adhesions, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

Insert

Paxillin

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
SLIRP.HMG6.wt.fl

Plasmid

#49821

Purpose

expression vector for MBP-SLIRP fusion

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

SLIRP SRA stem-loop interacting RNA binding protein (SLIRP Human)

Tags

E. coli Maltose Binding Protein

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-malG

Plasmid

#89951

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

malG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_FL

Plasmid

#59391

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/DH* (L213Q) uGFP

Plasmid

#14559

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F (Y142, Y160, Y174)/DH* (L213Q)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
QuasAr-P2A-GCaMP

Plasmid

#154082

Purpose

Independent expression of QuasAr and GCaMP under human synapsin 1 promoter

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

QuasAr-P2A-GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

Changed Tyrosine 327 to Alanine

Promoter

human synapsin 1

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAL950

Plasmid

#119748

Purpose

precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine

Tags

none

Expression

Bacterial

Mutation

Internal NdeI site removed and mutations Alanine …

Promoter

T7

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgYAP-2

Plasmid

#121423

Purpose

sgYAP-2 sequence: GAGATGACTTCCTGAACAGTG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgYAP-2

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-GW1D5(aa1670-1962)

Plasmid

#21519

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa1670-1962

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pMTH PKC epsilon

Plasmid

#8413

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C epsilon (Prkce Mouse)

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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