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pcDNA3.1-Gα13*-BERKY3

Plasmid

#158218

Purpose

BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-PRG(RH)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mScarlet-I-PXN

Plasmid

#112956

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

PXN

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pFW2000

Plasmid

#224211

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-flag-GFP-iCAM-1-Tether

Plasmid

#205187

Purpose

This vector encodes of a synCAM (iCAM1 Tether) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 tether and GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV4-p52

Plasmid

#23289

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

p52 (NFKB2 Human)

Expression

Mammalian

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EOS-S(4+)-EGFP

Plasmid

#21317

Purpose

Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

EOS-S(4+) promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pCA14-pHR-SFFV-GFP-RPA43

Plasmid

#199445

Purpose

Expression of GFP-RPA43 in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

RPA43 (POLR1F Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-flag-GFP-iCAM-1

Plasmid

#205186

Purpose

This vector encodes of a synCAM (iCAM1) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gαi*-BERKY3

Plasmid

#158198

Purpose

BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMaCTag-Z23

Plasmid

#120066

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Halo

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-14-3-3 ϵ

Plasmid

#48797

Purpose

expresses human myc-14-3-3ϵ in mammalian cells

Depositor

Huda Zoghbi

Article

Chen et al Cell. 2003 May 16;113(4):457-68.

Insert

YWHAE 14-3-3 ϵ (YWHAE Human)

Tags

haemagglutinin

Expression

Mammalian

Promoter

cmv

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pFH2.106_PB-EPN24-MCP_I-T2A-eGFP-PGK-Blast

Plasmid

#205551

Purpose

Express EPN24-MCP-I-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP-I

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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3xmNeonGreen_UtrCH

Plasmid

#129607

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Utrophin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE33

Plasmid

#90375

Purpose

AP1 - c-Fos, c-Jun, ATF, and JDP gene family gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

AP1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-DDX4N-mCh-Cry2WT

Plasmid

#101225

Purpose

DDX4(1-236) fused to mCh-Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

DDX4(1-236)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-flag-GFP-Ecad

Plasmid

#205188

Purpose

This vector encodes of a synCAM (Ecad) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM Ecad and GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P11

Plasmid

#120022

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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epi-BE4max

Plasmid

#135975

Purpose

Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Depositor

Yongming Wang

Article

Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-EFS-Puro

Plasmid

#227259

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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