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pcDNA3.1-SARS-CoV-2-S (Wuhan)

Plasmid

#215812

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMXSIP-CMTM6-HA

Plasmid

#204710

Purpose

expresses CMTM6-HA in mammalian cells

Depositor

William Kaelin

Article

Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

Insert

CMTM6 (CMTM6 Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEHAC1-CHIP-BirA-HA

Plasmid

#232587

Purpose

Express BirA tagged to the C-terminus of CHIP.

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

CHIP - BirA (CHIP Human)

Tags

HA

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_UAS-Ecad

Plasmid

#133804

Purpose

inducible expression of Ecad in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

Homo sapiens cadherin 1 (CDH1) (CDH1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-G-GECO1.2

Plasmid

#32446

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.2

Expression

Mammalian

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-SynB

Plasmid

#174861

Purpose

Expresses mouse syncytin B in mammalian cells

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

syncytin B (Synb Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSD117A

Plasmid

#218141

Purpose

Expresses AviTag-6xHis-MBP-TEV-SD40 for protein purification from E. coli

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD40

Tags

AviTag-6xHis-MBP-TEV-(GGGGS)2

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-HiBiT-N

Plasmid

#215815

Purpose

Expresses N protein with HiBiT tag in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 HiBiT Nucleocapsid (N )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCIG3 (pCMV-IRES-GFP version 3)

Plasmid

#78264

Purpose

Lentiviral empty cloning vector containing IRES and GFP

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV, lentiviral LTR

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-TadA8e-PcrA

Plasmid

#221228

Purpose

Express HACE Editor as a fusion of TadA-8e adenosine deaminase and PcrA helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Mm.cargo(Cre)

Plasmid

#174862

Purpose

Expresses mouse Peg10 cargoRNA encoding Cre recombinase

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

Cre flanked by MmPeg10 UTRs

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLIX402 PHF2

Plasmid

#91700

Purpose

inducible lentiviral expression of PHF2

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

PHF2 (PHF2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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NSP3-FR-2Strep

Plasmid

#210315

Purpose

Expresses SARS-CoV-2 NSP3 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP3 (ORF1ab SARS-CoV-2)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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U6-BbsI-GGsite-mCherry-Puro

Plasmid

#221232

Purpose

Cloning vector for sgRNA with mCherry and Puro

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Type

Empty backbone

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHelper-CBE-sgRNA

Plasmid

#221137

Purpose

Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

sgRNA-scaffold with Cas9 handle

Use

CRISPR

Expression

Bacterial

Promoter

J23119(SpeI)

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pConRef-2H12

Plasmid

#221182

Purpose

cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

Constitutive and None (same as cdGreen)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PRB

Plasmid

#89130

Purpose

mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-B (PGR Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEA038

Plasmid

#224547

Purpose

Blast-T2A-2xHA-FKPB12(dTagDegron) mouse Nipbl N-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

FKBP12F36V degron (dTAG system), blasticidin, 2xHA tag

Use

Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A

Plasmid

#41999

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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CapChR2_pmScarlet-N1

Plasmid

#188032

Purpose

Expresses CapChR2-mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR2-mScarlet

Tags

mScarlet

Expression

Mammalian

Mutation

S43D, L112C, T139C and N238E mutations in CoChR

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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