We narrowed to 15,732 results for: LEA

1. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-seq2

   Plasmid

   #240865

   Purpose

   GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes

   Depositor

   Mark Verheijen

   Article

   Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

   Insert

   U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
   

   Use

   AAV and CRISPR

   Tags

   SaCas9 + 3X HA-Tag

   Expression

   Mammalian

   Promoter

   GfaABC1D

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-Seq1

   Plasmid

   #240094

   Purpose

   GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes

   Depositor

   Mark Verheijen

   Article

   Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

   Insert

   U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
   

   Use

   AAV and CRISPR

   Tags

   SaCas9 + 3X HA-Tag

   Expression

   Mammalian

   Promoter

   GfaABC1D

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiCRISPR-B_2xsgRNAs_UPF3A/UPF3B (pAVA3129)

   Plasmid

   #239329

   Purpose

   Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting UPF3A and UPF3B

   Depositor

   Andrei Alexandrov

   Article

   Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

   Insert

   U6-driven sgRNA1 targting UPF3A and 7SK-driven sgRNA2 targeting UPF3B (UPF3B Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-GSDMD_H270A

   Plasmid

   #224701

   Purpose

   Expression of mutated Gasdermin D_H270A (GSDMD_H270A) in transfected mammalian cell lines

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD_H270A (GSDMD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H270A

   Promoter

   CMV

   Available Since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3.1-GSDMD_Q29A

   Plasmid

   #224702

   Purpose

   Expression of mutated Gasdermin D_Q29A (GSDMD_Q29A) in transfected mammalian cell lines

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD_Q29A (GSDMD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q29A

   Promoter

   CMV

   Available Since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3.1-GSDMD_Q29A_H270A

   Plasmid

   #224703

   Purpose

   Expression of mutant Gasdermin D_Q29A_H270A (GSDMD_Q29A_H270A) in transfected mammalian cell lines

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD_Q29A_H270A (GSDMD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q29A and H270A

   Promoter

   CMV

   Available Since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pcDNA3.1-GSDMD_D275A

   Plasmid

   #224704

   Purpose

   Expression of mutated Gasdermin D_D275A (GSDMD_D275A) in transfected mammalian cell lines

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD_D275A (GSDMD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D275A

   Promoter

   CVM

   Available Since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA3.1-GSDMD_H270A_D275A

   Plasmid

   #224705

   Purpose

   Expression of mutated Gasdermin D_H270A_D275A (GSDMD_D275A) in transfected mammalian cell lines

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD_Q29A_H270A (GSDMD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H270A and D275A

   Promoter

   CMV

   Available Since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCAG-MCP-Lbr-V5-mCherry

   Plasmid

   #235093

   Purpose

   Lbr mCherry fusion protein with MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-MCP-Lbr-V5-mCherry (LBR Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBIG1e-CDK12deltaNTD /Cyclin K

    Plasmid

    #231741

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK12 (CDK12 Human)
    CCNK (CCNK Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    CDK12(700-1490)

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBIG1e-CDK12deltaCTD/Cyclin K

    Plasmid

    #231742

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK12 (CDK12 Human)
    CCNK (CCNK Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    CDK12(1-1082)

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBIG1e-CDK12-KiD/Cyclin K

    Plasmid

    #231743

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK12 (CDK12 Human)
    CCNK (CCNK Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    CDK12(700-1082)

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-SpCas9-P2A-EGFP (MSP2582)

    Plasmid

    #223067

    Purpose

    pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized SpCas9-P2A-EGFP
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-multi-CRISPR-sgRipk1_#1-puro

    Plasmid

    #231984

    Purpose

    Knockout mouse Ripk1

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-multi-CRISPR-sgRipk1_#2-puro

    Plasmid

    #231983

    Purpose

    Knockout mouse Ripk1

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3xFLAG-DGKδ2-ΔSAMD

    Plasmid

    #226507

    Purpose

    Express N-terminal three tandem FLAG epitope tags, followed by an enterokinase cleavage site and human DGKD gene deleted SAM domain

    Depositor

    Chiaki Murakami, Fumio Sakane

    Article

    Murakami et al J Biol Chem. 2020 Mar 6;295(10):2932-294

    Insert

    DGKD (DGKD Human)
    

    Tags

    Three tandem FLAG epitope tag and enterokinase cl…

    Expression

    Mammalian

    Mutation

    deleted sterile alpha motif domain (deleted amino…

    Promoter

    CMV

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-FRB:GSlinker:SSD

    Plasmid

    #228535

    Purpose

    FRB protein in fusion with a bipartite SSD degron, consisting of fragments of human Skp1 and SOCS2 proteins, through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    FRB:GSlinker:SSD (SKP1, SOCS2 Human, Synthetic)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cdk13_intron3_sg4_pX458

    Plasmid

    #127339

    Purpose

    sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #4

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Mouse Cdk13 Intron 3 sgRNA (Cdk13 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Promoter

    U6 Promoter

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cdk13_intron4_sg3_pX330

    Plasmid

    #127340

    Purpose

    sgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #3

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Mouse Cdk13 Intron 4 Targeting sgRNA (Cdk13 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Promoter

    U6 Promoter

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR8/GW_TOPO_Cdk13_Untagged

    Plasmid

    #127342

    Purpose

    Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Mutation

    Base pairs 1-1554 of transgene are codon optimize…

    Promoter

    None

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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