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pMaCTag-P07

Plasmid

#124788

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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Galphai3-mTurquoise2

Plasmid

#69622

Purpose

The heterotrimeric G-protein subunit, Gαi isoform 3, tagged with mTurquoise2

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

Galphai3-mTurquoise2

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Rho*-BERKY3

Plasmid

#158220

Purpose

BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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CMVdel-dimericTomato-WASp(CRIB)

Plasmid

#191450

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WASp

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAM-CAGGS-FLEX-H2B-3XHA-P2A-N2c(G)

Plasmid

#73477

Purpose

Encodes the G protein to enable transsynaptic RAV infection

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

H2B-3XHA-P2A-N2c(G)

Use

AAV

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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(SUMO)10

Plasmid

#126948

Purpose

Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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dimericTomato-1xwGBD

Plasmid

#176099

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WAS (WAS Human)

Use

Lentiviral

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFW1000

Plasmid

#224210

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-GINKO1

Plasmid

#113111

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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mScarletI_MapTauC

Plasmid

#112964

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

MapTauC

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos

Plasmid

#211840

Purpose

Plant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

HA and mCherry

Expression

Plant

Promoter

UBIQUITIN10

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-HuR

Plasmid

#121162

Purpose

Mammalian expression of GFP tagged HuR

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

HuR (ELAVL1 )

Expression

Mammalian

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-LNGFR-recipient

Plasmid

#11660

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

LNGFR

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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PL-SIN-Nanog-EGFP

Plasmid

#21321

Purpose

Lentiviral vector, expresses EGFP under Nanog promoter

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

Nanog promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z02

Plasmid

#120045

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TagBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TagBFP

Use

Pcr template

Tags

TagBFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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3xnls-mTurquoise2

Plasmid

#98817

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-UtrCH

Plasmid

#98823

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

UtrCH

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-PGK-Puro

Plasmid

#227264

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z13

Plasmid

#120056

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry

Use

Pcr template

Tags

mCherry

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag Rac2

Plasmid

#12192

Depositor

Gary Johnson

Article

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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