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pHR myc-MBP-SspB-iRFP-NLS

Plasmid

#223286

Purpose

An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

Insert

Myc-MBP-SspB-irFP-NLS

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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MGS0156_pET29b

Plasmid

#215410

Purpose

Expression construct for MGS0156 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

MGS0156

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJPT001-HaloTag-1x FLAG

Plasmid

#202492

Purpose

Express "soluble" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Tags

1x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Ani- α Amylase SP

Plasmid

#153503

Purpose

Aspergillus niger α Amylase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

α Amylase SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_ATP1.0

Plasmid

#167579

Purpose

Express the ATP sensor GRAB_ATP1.0 in astrocytes

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Promoter

GfaABC1D

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHCMV_RV_G

Plasmid

#207303

Purpose

Mammalian expression of Rabies virus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

RV_G (RABVgp4 Rabies virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold(c4)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pmScarlet_C1

Plasmid

#85042

Purpose

Mammalian expression of mScarlet

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV4-Tax WT

Plasmid

#23284

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

Tax

Expression

Mammalian

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only

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pBAD-6xHis-AviTag-ALFAtag-eGFP

Plasmid

#195854

Purpose

Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

6xHis-AviTag-ALFAtag-eGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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anti-ALFA_Nb_GFP

Plasmid

#244063

Purpose

fusion of the ALFA binder (nanobody) to sfGFP

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

anti-ALFA_Nb_sfGFP_GB1

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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Vimentin_mCherry_ALFA

Plasmid

#244059

Purpose

anchoring mCherry-1x ALFA to the intermediate filaments

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

NtVimentin-mCherry-ALFA

Tags

ALFA

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pG-HIV-2LTR

Plasmid

#104590

Purpose

standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

2LTR circle junction of HIV-1

Use

Cloning vector

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMOS028: Mitycam calcium sensor (mitochondrial)

Plasmid

#163046

Purpose

Mitycam calcium sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Mitycam

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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eMagB-TgRFP-T

Plasmid

#162252

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagB-TgRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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eMagBF-TgRFPT

Plasmid

#162253

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagBF-TgRFPT

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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eMagA-EGFP-Mito

Plasmid

#162244

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagA-EGFP-OMP25

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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eMagAF-EGFP-Mito

Plasmid

#162243

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagAF-EGFP-OMP25

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.EF1a.FLEX.MTRIA-OT

Plasmid

#184595

Purpose

For production of AAV for a fluorescent sensor for oxytocin

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OT

Use

AAV

Promoter

Human elongation factor-1 alpha promoter

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQAT04

Plasmid

#223376

Purpose

T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-SpCas9-OsU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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