We narrowed to 22,434 results for: ESC;

1. pLL3.7 Lmo4.2 shRNA

   Plasmid

   #59293

   Purpose

   shRNA to mouse Lmo4

   Depositor

   Ulrike Heberlein

   Article

   Lasek et al Genes Brain Behav. 2010 Oct;9(7):817-24.

   Insert

   Lmo4.2 shRNA
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pLL3.7 Lmo4.3 shRNA

   Plasmid

   #59292

   Purpose

   shRNA to mouse Lmo4

   Depositor

   Ulrike Heberlein

   Article

   Lasek et al Genes Brain Behav. 2010 Oct;9(7):817-24.

   Insert

   Lmo4.3 shRNA
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

3. P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p)

   Plasmid

   #70210

   Purpose

   1 Part of 4-part switch

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   deGFP
   

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. cisRAJ31-GFP-LAA

   Plasmid

   #69928

   Purpose

   Expresses cisRAJ31-GFP-LAA, a cis-repressed mRNA regulated by RAJ31.

   Depositor

   Alfonso Jaramillo

   Article

   Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

   Insert

   PllacO1-cisRAJ31-GFP-LAA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PlLacO1

   Available Since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. circRAJ31-Toehold_mutant

   Plasmid

   #69930

   Purpose

   Expresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.

   Depositor

   Alfonso Jaramillo

   Article

   Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

   Insert

   plTet-circRAJ31_toeholdmutant
   

   Use

   Synthetic Biology

   Mutation

   4 bases mutated in toehold of riboregulator

   Promoter

   PltetO1-2

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Aurein1.2_(+36GFP)-BirA

   Plasmid

   #71752

   Purpose

   Aurein1.2+36GFP-BirA fusion for E.Coli expression

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   Aurein1.2+36GFP-BirA
   

   Tags

   6XHis

   Expression

   Bacterial

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pANY3

   Plasmid

   #112197

   Purpose

   This plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.

   Depositor

   Yingfeng An

   Article

   Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

   Type

   Empty backbone

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJDC122

   Plasmid

   #157876

   Purpose

   tdTomato is expressed under the mycobacterial phsp60 heat shock promoter for in vivo and in vitro detection.

   Depositor

   Jeffrey Cirillo

   Article

   Kong et al PLoS One. 2016 Mar 2;11(3):e0149972. doi

   Insert

   tdTomato
   

   Expression

   Bacterial

   Available Since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGE3

   Plasmid

   #79442

   Purpose

   recipient plasmid [multiplexing]; pPcUbi:Cas9, nptII

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGE64

    Plasmid

    #79446

    Purpose

    recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGE1

    Plasmid

    #79440

    Purpose

    recipient plasmid [multiplexing]; p35S:Cas9, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJ414 - DANCER-3

    Plasmid

    #136387

    Purpose

    Expresses DANCER-3 dynamic protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    DANCER-3
    

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V39L-V54I

    Promoter

    T7 promoter

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLNL1134

    Plasmid

    #160865

    Purpose

    PJ23110-RBS-ScEHT1-mRuby2-tL3S2P24_KanR_p15A

    Depositor

    John Dueber

    Article

    Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

    Insert

    Medium-chain fatty acid ethyl ester synthase
    

    Use

    Synthetic Biology

    Tags

    mRuby2

    Expression

    Bacterial

    Promoter

    PJ23110

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCerulean3-LC-Myosin-C-10

    Plasmid

    #55427

    Purpose

    Localization: Light Chain Myosin, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    LC-Myosin
    

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

    Plasmid

    #162810

    Purpose

    STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    BsmBI_Intein_DD
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS1_4x7SKPylT_EF1_Ub-*GFP

    Plasmid

    #162806

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled GFP

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    EF1_Ub-(N-TAG)GFP
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    GFP (Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. SepOTSγ

    Plasmid

    #68284

    Purpose

    Phosphoserine orthogonal translation system

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS9/EF-Sep21, 1xtRNA-Sep
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lifeact-rsLOV1 pcDNA3.1(+)

    Plasmid

    #107881

    Purpose

    Mammalian expression of Lifeact-rsLOV1

    Depositor

    Stefan Hell

    Article

    Gregor et al Sci Rep. 2018 Feb 9;8(1):2724. doi: 10.1

    Insert

    rsLOV1
    

    Tags

    Lifeact

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19-P70a-Bottom-T

    Plasmid

    #87313

    Purpose

    Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system "Bottom" transcript
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNH078

    Plasmid

    #42164

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    mTFP1
    

    Expression

    Worm

    Available Since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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