Addgene

We narrowed to 21,567 results for: ESC;

Showing: 8081 - 8100 of 21567 results

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pNB776

Plasmid

#60117

Purpose

Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

RdLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Mutation

Promoter

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNH030

Plasmid

#42149

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1::linker::2xNLS

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pGST1-yGGA1-VHS

Plasmid

#44421

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yGGA1-VHS (GGA1 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
P4U2C6T2-v1-2 (Pl-tetO1-lacI) (137p)

Plasmid

#70213

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

lacI

Use

Tags

Expression

Mutation

Promoter

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE65

Plasmid

#79447

Purpose

recipient plasmid [single nuclease]; pPcUbi:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE146

Plasmid

#79449

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE167

Plasmid

#79457

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE168

Plasmid

#79458

Purpose

recipient plasmid [multiplexing]; pDD45:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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tdTomato-Cx43-7

Plasmid

#58086

Purpose

Localization: Gap Junctions, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Cx43

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
EC.RNe.TYB1.a1-395.wt

Plasmid

#17950

Depositor

David McKay

Article

Caruthers et al J Biol Chem. 2006 Sep 15. 281(37):27046-

Insert

Escherichia coli RNase E residues 1-395

Use

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

residues 1-395

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
SepOTSν

Plasmid

#68294

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 5xtRNA-Sep

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSβ

Plasmid

#68283

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 1xtRNA-Sep

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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LI B-C N-NES KOZ b c7

Plasmid

#54445

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Nuclear Export Signal (NES) of heat stable inhibitor (PKI)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoMaxGFP-Kan

Plasmid

#44907

Purpose

Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoMaxGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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mPlum-Alpha-Actinin-19

Plasmid

#55949

Purpose

Localization: alpha Actinin, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

Alpha-Actinin (ACTN1 Human)

Use

Tags

mPlum

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only
mEos2-LaminA-C-18

Plasmid

#57394

Purpose

Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

LaminA (LMNA Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLL3.7 Alk.1 shRNA

Plasmid

#59296

Purpose

shRNA to mouse Alk

Depositor

Ulrike Heberlein

Article

Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41.

Insert

Alk.1 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pDR-meptrac-H194E

Plasmid

#47764

Purpose

expresses MepTrac-H194E in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

MepTrac-H194E

Use

Tags

mcpGFP

Expression

Yeast

Mutation

H194E

Promoter

PMA

Available since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pDR-AmTrac-100

Plasmid

#47771

Purpose

expresses AmTrac-100 in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac-100

Use

Tags

mcpGFP

Expression

Yeast

Mutation

A141E, T464D

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pCS2 xenopus twisted gastrulation

Plasmid

#24974

Depositor

Edward De Robertis

Article

Larrain et al Development. 2001 Nov . 128(22):4439-47.

Insert

twisted gastrulation (Twsg1 Mouse)

Use

Tags

FLAG and chordin signal peptide

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only