159,461 results

1. tetO-FOSL1

   Plasmid

   #170687

   Purpose

   doxycycline-inducible overexpression of FOSL1

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   FOSL1 (FOSL1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p4455 FLAG-hPLIC-2

   Plasmid

   #8661

   Depositor

   Peter Howley

   Article

   Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

   Insert

   hPLIC-2 (UBQLN2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-BAZ2A

   Plasmid

   #65373

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BAZ2A (BAZ2A Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF0799

   Plasmid

   #141705

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ATF3 (ATF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRsetB-PercevalHR

   Plasmid

   #49081

   Purpose

   Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Use

   Tags

   7xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a_MYCN_P2A_Hygro_Barcode

   Plasmid

   #120463

   Purpose

   Barcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYCN (MYCN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH2B-miRFP703

   Plasmid

   #80001

   Purpose

   Histone 2B labeling with near-infrared fluorescent protein miRFP703

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   histone cluster 1 H2B family member j (H2BC11 Human)
   

   Use

   Tags

   miRFP703

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS418

   Plasmid

   #11256

   Depositor

   Fred Cross

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-Dvl2-GFP

   Plasmid

   #109148

   Purpose

   Stable Dvl2 expression

   Depositor

   Mariann Bienz

   Article

   Gammons et al J Cell Sci. 2016 Oct 15;129(20):3892-390

   Insert

   Dvl2 (DVL2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. M23: pHR-SFFVp-FUSN::mCherry::SspB

    Plasmid

    #122148

    Purpose

    N terminal domain of FUS (1-214) tagged with SspB and mCherry.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FUS (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-sspB

    Expression

    Mammalian

    Mutation

    Contains only amino acids 1-214

    Promoter

    SFFV

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENN226_CRISPRcharm

    Plasmid

    #220834

    Purpose

    CRISPRcharm expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    CRISPRcharm
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-mCherry-IRES-Dre

    Plasmid

    #55633

    Purpose

    Expresses Dre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Inserts

    mCherry
    Dre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a and Ef1a/IRES

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-Cas9-T2A-GFP

    Plasmid

    #63155

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST-RH▲-MNase

    Plasmid

    #136292

    Purpose

    Expression of catalytic inactive RNase H fused to micrococcal nuclease

    Depositor

    Kavitha Sarma

    Article

    Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

    Insert

    RNase HI-D10R-E48R and MNase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6b:sgRNA#3

    Plasmid

    #64247

    Purpose

    expresses sgRNA under U6b promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTagRFP657-C1

    Plasmid

    #31872

    Purpose

    For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.

    Depositor

    Vladislav Verkhusha

    Article

    Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

    Insert

    TagRFP657
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET30ax

    Plasmid

    #85761

    Purpose

    A bacterial expression vector with a His-tag at N and C-terminus.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Type

    Empty backbone

    Use

    Tags

    His tag (N-term and C-term)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. shCD44-2 pRRL

    Plasmid

    #19123

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against CD44 (CD44 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1.sh.beta-catenin.1248

    Plasmid

    #19761

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    small hairpin RNA against beta-catenin (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. CL20_mEGFP_ETV6_RUNX1

    Plasmid

    #205818

    Purpose

    Express mEGFP-tagged fusion protein, ETV6_RUNX1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    ETV6_RUNX1 (ETV6, RUNX1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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