We narrowed to 12,356 results for: nsf

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pRPN265NB-enh-MNase

Plasmid

#166035

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Article

Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

Insert

Glutathione-S-Transferase/enhancer/MNase

Tags

GST

Expression

Bacterial

Promoter

lac promoter / tac promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(H134R)-EYFP

Plasmid

#26969

Purpose

AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

Has Service

AAV1, AAV2, AAV5, and AAV9

Insert

hChR2(H134R)

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Histidine 134 is mutated to Arginine

Promoter

CaMKIIa

Available Since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

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sUPRa

Plasmid

#223242

Purpose

sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

Depositor

Colin Akerman

Article

Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

Insert

sUPRa

Use

AAV

Promoter

Custom

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC cloning vector

Plasmid

#10878

Purpose

3rd generation transfer plasmid

Depositor

David Root

Article

Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-OsTIR1(F74G)

Plasmid

#140730

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Use

AAV

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mScarlet

Plasmid

#131001

Purpose

AAV vector to drive the expression of mScarlet under the control of human Synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV1 and AAV8

Insert

mScarlet

Use

AAV

Promoter

hSyn

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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CDK1as_T2A_puromycin

Plasmid

#118595

Purpose

One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

CDK1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGFP-Hyg-CTCF-N1

Plasmid

#131799

Purpose

Expresses human CTCF-GFP in mammalian cells through transient transfection

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

CTCF (CTCF Human)

Tags

GFP

Expression

Mammalian

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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RSV-Flag-Brd4

Plasmid

#86616

Purpose

Flag tagged mouse Brd4 expression construct for cell transfection

Depositor

Mario García-Domínguez

Article

Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

Insert

Brd4 (Brd4 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

RSV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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CDK1as_T2A_Zeo

Plasmid

#118596

Purpose

One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

CDK1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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AAV GFP

Plasmid

#49055

Purpose

adeno-associated virus (AAV) encoding a green fluorescent protein

Depositor

Fred Gage

Article

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hALDOA

Plasmid

#210809

Purpose

For transient transfection

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-SypHer2-DAAO-NES

Plasmid

#119184

Purpose

Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

Depositor

Thomas Michel

Article

Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

Insert

SypHer2-DAAO-NES

Use

AAV

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-Cre-GFP

Plasmid

#68544

Purpose

AAV expression of Cre-GFP fusion

Depositor

Eric Nestler

Article

Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

Insert

Cre

Use

AAV and Cre/Lox

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-mScarlet

Plasmid

#131000

Purpose

AAV vector to drive the expression of mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

mScarlet

Use

AAV

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgRNA

Plasmid

#124844

Purpose

Vector for Cre-dependent expression of SaCas9

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SaCas9, gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS and NLS-3xHA

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GCaMP6s.WPRE.SV40

Plasmid

#100844

Purpose

AAV expression of ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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MP9956:SFG.GD3synthase-2A-GD2synthase

Plasmid

#75013

Purpose

Retroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.

Depositor

Martin Pule

Article

Thomas et al PLoS One. 2016 Mar 31;11(3):e0152196. do

Inserts

murine GD3 synthase
murine GD2 synthase

Use

Retroviral; Momlv retroviral vector

Expression

Mammalian

Available Since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-eGFP-KASH

Plasmid

#248781

Purpose

AAV construct expressing eGFP-KASH enabling FACS-sorting of extracted nuclei

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

eGFP-KASH

Promoter

Cbh

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gnaq

Plasmid

#246001

Purpose

Express HA N-terminal tagged mouse Galphaq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnaq (Gnaq Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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