We narrowed to 866 results for: seph

1. pPSV39-ugi

   Plasmid

   #186556

   Purpose

   Broad host range inducible expression of uracil-DNA glycosylase inhibitor (UGI)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   ugi
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LacUV5

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPSV39-dddAI

   Plasmid

   #186557

   Purpose

   Broad host range regulated expression of the DddA immunity determinant (DddAI)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   dddAI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-mCep290

   Plasmid

   #27379

   Depositor

   Joseph Gleeson

   Article

   Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

   Insert

   Cep290 (Cep290 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FLAG-mCep290

   Plasmid

   #27381

   Depositor

   Joseph Gleeson

   Article

   Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

   Insert

   Cep290 (Cep290 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-short_BrokenHeart (no BC)

   Plasmid

   #86950

   Purpose

   AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   Brokenheart construct
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCIG AKT3 E17K

   Plasmid

   #73050

   Purpose

   Human AKT3 E17K

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   AKT3 E17K (AKT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E17K

   Promoter

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-rBrokenheart

   Plasmid

   #203394

   Purpose

   AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

   Insert

   Brokenheart
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG mFoxg1-GFP wildtype

   Plasmid

   #73048

   Purpose

   C-terminal fusion of gfp Mouse wildtype Foxg1

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   mFoxg1 (Foxg1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-TEV-U1AF37MF77M

   Plasmid

   #168267

   Purpose

   Expresses human dmU1A with the F37M/F77M double mutant

   Depositor

   Joseph Wedekind

   Article

   Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

   Insert

   U1A RRM1 crystallization module (SNRPA Human)
   

   Use

   Tags

   6xHis tag N-terminus, TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   changed F37M and F77M

   Promoter

   T7 promotor

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIG AKT3 K177M

    Plasmid

    #73051

    Purpose

    Human AKT3 K177M

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    AKT3 K177M (AKT3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K177M

    Promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCIG AKT3 S472A

    Plasmid

    #73052

    Purpose

    Human AKT3 S472A

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    AKT3 S472A (AKT3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S472A

    Promoter

    Available since

    March 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCIG AKT3 S472E

    Plasmid

    #73053

    Purpose

    Human AKT3 S472E

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    AKT3 S472E (AKT3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid

    #133793

    Purpose

    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    CjeCas9 sgRNA entry vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V2-TGAA linker sgRNA architecture from Kim et al.…

    Promoter

    U6

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-human-BNIP3 delta Exon2

    Plasmid

    #100782

    Purpose

    Mammalian expression of BNIP3 delta Exon2 splice variant

    Depositor

    Joseph Gordon

    Article

    Field et al Cell Death Discov. 2018 Sep 21;4:37. doi

    Insert

    human-BNIP3 delta Exon2 (BNIP3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Exon2 deletion

    Promoter

    CMV

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pfast bac cry1myc

    Plasmid

    #51892

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    cry1 (Cry1 Mouse)
    

    Use

    Tags

    3x Flag and myc

    Expression

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pfast bac Rbx-HA

    Plasmid

    #51893

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    Rbx1 (RBX1 Human)
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

17. HLH Bmal Pas 1-465-VenC I317D

    Plasmid

    #47367

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, I317D

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pCAG mFoxg1-GFP T271E

    Plasmid

    #73057

    Purpose

    C-terminal GFP fusion of Mouse Foxg1 T271E

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    Foxg1 (Foxg1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T271E

    Promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG mFoxg1-GFP T271A

    Plasmid

    #73047

    Purpose

    C-terminal fusion of gfp Mouse Foxg1 T271A

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    Foxg1 (Foxg1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T271A

    Promoter

    CAG

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS10-riboE-dddAI

    Plasmid

    #186561

    Purpose

    Regulated expression of the DddA immunity determinant (DddAI) in Firmicutes

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    dddAI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pSpac(hy)

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge