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We narrowed to 17,814 results for: STO

Showing: 1201 - 1230 of 17814 results
  1. pDEST-Spe4-RfA-mScarlet-I-stop

    Plasmid
    #186393
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

    Plasmid
    #127356
    Purpose
    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins
    Depositor
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and mVenus
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. EK0379 CMV-TO-EGFP-stop-t70587 (PB)

    Plasmid
    #191154
    Purpose
    Expression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.
    Depositor
    Insert
    EGFP-t70587
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV-TO
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pDEST-Spe4-RfA-Halotag-stop

    Plasmid
    #186387
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Halo tag into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pENTR_L1-miniTurbo-NES-YFP-STOP-L2

    Plasmid
    #127359
    Purpose
    gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice
    Depositor
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

    Plasmid
    #68345
    Purpose
    A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression
    Inserts
    puromycin
    hCas9
    Use
    CRISPR; Flp/frt
    Tags
    linked to red flouresent protein gene mKateII and…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. pENT-L1-Kozak-EGFP-GPI-stop-L2

    Plasmid
    #186355
    Purpose
    Entry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequences
    Depositor
    Insert
    signal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site (CD59 Human, Synthetic)
    Use
    Expression of a fluorescent membrane marker
    Tags
    EGFP
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 1201 - 1230 of 17814 results