Addgene

We narrowed to 13,557 results for: TIM

Showing: 2001 - 2050 of 13557 results

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CeNL/pcDNA3

Plasmid

#85199

Purpose

Cyan color variant of bright luminescent protein

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

Cyan enhanced Nano-lantern

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-MCH

Plasmid

#184606

Purpose

For expression of a fluorescent sensor for melanin-concentrating hormone in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-MCH

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHCMV-RabiesG

Plasmid

#15785

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

Rabies virus G protein (RABVgp4 Rabies virus (strain SAD B19))

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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GeNL(Ca2+)_520/pcDNA3

Plasmid

#85204

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_520

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-ATP

Plasmid

#184599

Purpose

For expression of a fluorescent sensor for ATP in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-ATP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP-T-Kan

Plasmid

#44906

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP-T

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoSuperfolderGFP-SpHis5

Plasmid

#44837

Purpose

Yeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoSuperfolderGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_Beta4Gal-HaloTag7

Plasmid

#175536

Purpose

Expression of HaloTag7 at the at the Gogli apparatus of the mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Beta4Gal-HaloTag7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCE1

Plasmid

#174434

Purpose

Candida optimized monomeric eGFP with RIPLING linker sequence.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

Candida optimized monomeric GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

Plasmid

#175529

Purpose

Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Cox8a-HaloTag7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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GeNL(Ca2+)_60/pcDNA3

Plasmid

#85207

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_60

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pUCBB-oriT

Plasmid

#32556

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

oriT

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pME-nfsB-CFP

Plasmid

#86374

Purpose

expression of nfsB-CFP

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

nitroreductase

Use

Zebrafish

Tags

ECFP

Expression

Mutation

Promoter

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaD (pESC106)

Plasmid

#50388

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Erick Strauss, Tadhg Begley

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

PPAT

Use

Tags

6xHis-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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OeNL/pcDNA3

Plasmid

#85202

Purpose

Orange color variant of bright luminescent protein

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

Orange enhanced Nano-lantern

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only
pSfinx-mYFP

Plasmid

#216206

Purpose

A simplified disease resistance assay using YFP-expressing Potato Virus X in N. benthamiana

Depositor

Takaki Maekawa

Article

Hasegawa et al Plant Physiol. 2024 Jul 8:kiae368. doi:

Insert

monomeric yellow fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_b4g-HaloTag9

Plasmid

#175546

Purpose

Expression of HaloTag9 at the at the Gogli apparatus of the mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Beta4Gal-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJKR-O-mphR

Plasmid

#62570

Purpose

Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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tdTomato-Lifeact-7

Plasmid

#54528

Purpose

Localization: Actin, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Type

Empty backbone

Use

Tags

tdTomato-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pMOS017: ratiometric pHluorin (cytosolic)

Plasmid

#163052

Purpose

ratiometric pHluorin (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMIG-Aalpha WT

Plasmid

#10884

Depositor

William Hahn

Article

Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

Insert

PP2A Aalpha (PPP2R1A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pHFS:SEP-rGluA1 TKIT donor

Plasmid

#169438

Purpose

Donor DNA for TKIT knockin of SEP to GluA1 in rat

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pmNeonGreenHO-mito

Plasmid

#127913

Purpose

a mammalian expression vector for mitochondria-targeting humanized mNeonGreen

Depositor

Isei Tanida

Article

Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

Insert

humanized mNeonGreen with mitocondrial targetting signal

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-Ec.coaE (pESC124)

Plasmid

#50390

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Erick Strauss, Tadhg Begley

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

DPCK

Use

Tags

6XHIS tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB_PalkB_sfgfp_KanR

Plasmid

#166503

Purpose

Expression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.

Depositor

Sven Panke

Article

Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PalkB

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-PCNA-IRES-puro2b

Plasmid

#26461

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

PCNA (PCNA Human)

Use

Tags

pEGFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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ERoxBFP

Plasmid

#68126

Purpose

mammalian expression of ER localized oxBFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Insert

oxBFP

Use

Tags

KDEL ER retention sequence and bovine prolactin s…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

Plasmid

#175534

Purpose

Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

IgKchL_HA-HaloTag7-myc_PDGFRtmb

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoEGFP-CaURA3

Plasmid

#44872

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
CIp10 - mTurquoise2 (γmGFP based)

Plasmid

#163121

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

mTurquoise2 - γmGFP based

Use

Candida albicans expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-CyRFP1

Plasmid

#84356

Purpose

Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

CyRFP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p177bp-LacOx162-Hyg

Plasmid

#210215

Purpose

Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

162xLacO repeats derived from: PMID: 12864855.

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YeNL_pRSETb

Plasmid

#89520

Purpose

Yellow color variant of bright luminescent protein for bacterial expression

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Use

Tags

His X 6

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoEGFP-SpHis5

Plasmid

#44836

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pJKR-H-cdaR

Plasmid

#62557

Purpose

Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Inserts

beta lactamase
sfGFP
CdaR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-oChIEF-tdTomato

Plasmid

#50977

Purpose

AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mutation

ChIEF gene is mammalian codon optimized

Promoter

human synapsin promoter

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMiniT:GFP-Gephyrin TKIT donor

Plasmid

#169433

Purpose

Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pG-HIV-2LTR

Plasmid

#104590

Purpose

standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

2LTR circle junction of HIV-1

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.Syn.MTRIA-OT

Plasmid

#184594

Purpose

For production of AAV for a fluorescent sensor for oxytocin

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OT

Use

AAV

Tags

Expression

Mutation

Promoter

Human synapsin promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LT3GEN

Plasmid

#111173

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

T3G

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMiniT:SEP-GluA1 TKIT donor

Plasmid

#169418

Purpose

Donor DNA for TKIT knockin of SEP to GluA1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomCherry-CaURA3

Plasmid

#44876

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mCherry with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
csr-1_son-1_mNeonGreen

Plasmid

#191743

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Use

Tags

NCU04502 promoter and mNeonGreen

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHARGE3

Plasmid

#24658

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

Turbo

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
ICP8-P2A/Crimson (pSLIK4)

Plasmid

#113859

Purpose

For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

ICP8

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB-XIAPmut

Plasmid

#11559

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAPmut (XIAP Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed his 467 to Alanine

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
TroponinT-GCaMP5-Zeo

Plasmid

#46027

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GCaMP5-2A-Zeo

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TNNT2

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-BICD2(1-500)-FRB

Plasmid

#174648

Purpose

Rapalog-inducible coupling to dynein adaptor BICD2

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

HA-BICD2(1-500)-FRB

Use

Tags

FRB and HA

Expression

Mammalian

Mutation

mmBICD2(aa1-500): Lys213Glu

Promoter

Chicken beta-actin

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-MTN

Plasmid

#184607

Purpose

For expression of a fluorescent sensor for melatonin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-MTN

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pH_35S/mEGFP

Plasmid

#135321

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed hygromycin phosphotransferase selectable marker (hpt)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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