We narrowed to 2,540 results for: organization Addgene

1. pLenti-BE3-P2A-Puro

   Plasmid

   #110838

   Purpose

   Lentiviral vector for constitutive expression of BE3 (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3
   

   Use

   Lentiviral

   Mutation

   D10A

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-BE3-FNLS

   Plasmid

   #110836

   Purpose

   CMV expression vector for BE3-FNLS construct (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3-FNLS
   

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   NLS sequence at the N-terminus

   Promoter

   CMV

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TTR F87E

   Plasmid

   #238075

   Purpose

   Bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

   Insert

   TTR (TTR Human)
   

   Expression

   Bacterial

   Mutation

   F87E

   Promoter

   T7

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Scon_PheRS_TP_pK7fWG2_IVD_FP611

   Plasmid

   #228372

   Purpose

   Expresses N-term transit peptide from Silene conica PheRS (Warren et al., 2023) with demonstrated plastid targeting; proof-of-concept for Plasmid 228371

   Depositor

   Daniel Sloan

   Article

   DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   N-terminal transit peptide from Silene conica PheRS (Warren et al., 2023) with demonstrated plastid targeting.
   

   Tags

   Green Florescent Protien

   Expression

   Plant

   Available Since

   Feb. 26, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611

   Plasmid

   #228374

   Purpose

   Expresses N-term transit peptide from Sapria himalayana Aspartate aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3212.91; determination of S. himalayana AspRS target localization

   Depositor

   Daniel Sloan

   Article

   DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   N-terminal transit peptide from S. himalayana AspRS evm.model.fragScaff scaffold 3212.91
   

   Tags

   Green Florescent Protien

   Expression

   Plant

   Available Since

   Feb. 26, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. Shim_CytC_87.18_SHI04112_TP_GFP_pK7fWG2_IVD_FP611

   Plasmid

   #228375

   Purpose

   Expresses N-term transit peptide from Sapria himalayana Cytochrome C with demonstrated mito targeting; proof-of-concept for Plasmid 228371

   Depositor

   Daniel Sloan

   Article

   DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   N-terminal transit peptide from S. himalayana Cytochrome C with demonstrated mito targeting.
   

   Tags

   Green Florescent Protien

   Expression

   Plant

   Available Since

   Feb. 26, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. Shim_GluRS_87.31.3_TP_GFP_pK7fWG2_IVD_FP611

   Plasmid

   #228376

   Purpose

   Expresses N-term transit peptide from Sapria himalayana Glutamate aminoacyl-tRNA synthetase evm.model.fragScaff_scaffold_87.31.3; determination of S. himalayana GluRS target localization

   Depositor

   Daniel Sloan

   Article

   DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   N-terminal transit peptide from S. himalayana GluRS evm.model.fragScaff_scaffold_87.31.3
   

   Tags

   Green Florescent Protien

   Expression

   Plant

   Available Since

   Feb. 26, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. Shim_MetRS_440.109_TP_GFP_pK7fWG2_IVD_FP611

   Plasmid

   #228378

   Purpose

   Expresses N-term transit peptide from Sapria himalayana Methionine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 440.10; determination of S. himalayana MetRS target localization

   Depositor

   Daniel Sloan

   Article

   DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   N-terminal transit peptide from S. himalayana MetRS evm.model.fragScaff scaffold 440.10
   

   Tags

   Green Florescent Protien

   Expression

   Plant

   Available Since

   Feb. 26, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. Shim_ThrRS_3895.3_TP_GFP_pK7fWG2_IVD_FP611

   Plasmid

   #228380

   Purpose

   Expresses N-term transit peptide from Sapria himalayana Threonine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3895.3; determination of S. himalayana ThrRS target localization

   Depositor

   Daniel Sloan

   Article

   DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   N-terminal transit peptide from S. himalayana ThrRS evm.model.fragScaff scaffold 3895.3
   

   Tags

   Green Florescent Protien

   Expression

   Plant

   Available Since

   Feb. 26, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. Shim_TrpRS_2406.7.1_SHI16564_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid

    #228381

    Purpose

    Expresses N-term transit peptide from Sapria himalayana Tryptophan aminoacyl-tRNA synthetase (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA); to determine S. himalayana TrpRS target localization

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from S. himalayana TrpRS (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA)
    

    Tags

    Green Florescent Protien

    Expression

    Plant

    Available Since

    Feb. 26, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pKT2/CAGXSP/GFPCD63

    Plasmid

    #231709

    Purpose

    Stably express CD63 fused with GFP in mammalian cells via the sleeping beauty transposon system.

    Depositor

    Masamitsu Kanada

    Article

    Li et al Sci Rep. 2024 Nov 9;14(1):27432. doi: 10

    Insert

    CD63 (CD63 Human)
    

    Tags

    gfp

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP_A893E-talin1 (1-2541)

    Plasmid

    #166116

    Purpose

    For expression of full-length mouse talin-1 carrying mutation A893E in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    A893E-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A893E

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP_L1539P-talin1 (1-2541)

    Plasmid

    #166119

    Purpose

    For expression of full-length mouse talin-1 carrying mutation L1539P in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    L1539P-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L1539P

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP_L2509P-talin1 (1-2541)

    Plasmid

    #166123

    Purpose

    For expression of full-length mouse talin-1 carrying mutation L2509P in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    L2509P-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L2509P

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP_S1750F-talin1 (1-2541)

    Plasmid

    #166120

    Purpose

    For expression of full-length mouse talin-1 carrying mutation S1750F in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    S1750F-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S1750F

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP_D2086V-talin1 (1-2541)

    Plasmid

    #166122

    Purpose

    For expression of full-length mouse talin-1 carrying mutation D2086V in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    D2086V-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D2086V

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP_R1368W-talin1 (1-2541)

    Plasmid

    #166117

    Purpose

    For expression of full-length mouse talin-1 carrying mutation R1368W in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    R1368W-talin1-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R1368W

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP_∆DD-talin1 (1-2493)

    Plasmid

    #166124

    Purpose

    For expression of mouse talin-1 (residues 1-2493) containing C-terminal deletion of dimerization domain (∆DD) in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    ∆DD-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    residues 1-2493, deletion of dimerization domain …

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP_Y1389C-talin1 (1-2541)

    Plasmid

    #166118

    Purpose

    For expression of full-length mouse talin-1 carrying mutation Y1389C in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    Y1389C-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Y1389C

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3/flag-pBirA(1-K283)-FRB

    Plasmid

    #154451

    Purpose

    expresses flag-pBirA(1-K283)-FRB/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    flag-pBirA(1-K283)-FRB
    

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pmCherry C1 Omp25

    Plasmid

    #157758

    Purpose

    Expression of mcherry-labelled mitochondrial outer membrane protein 25 in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    SYNJ2bp (SYNJ2BP Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

    Plasmid

    #91881

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

23. AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

    Plasmid

    #91882

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

24. PMP34-mApple

    Plasmid

    #214417

    Purpose

    Peroxisome localization of PMP34 fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    PMP34 (SLC25A17 Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP

    Plasmid

    #201681

    Purpose

    Lentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expression

    Depositor

    Emma Rawlins

    Article

    Lim et al bioRxiv [Preprint]. 2023 Sep 4:2023.08.3

    Inserts

    SFTPC promoter region (2kb upstream)
    TagRFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TRPML1-G-GECO1.2-ERES

    Plasmid

    #207144

    Purpose

    Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    Mcoln1 (MCOLN1 Human)
    

    Tags

    ER-Export Signal (ERES) and G-GECO1.2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV AURKA-mTurq2

    Plasmid

    #157767

    Purpose

    Expression of AuroraA kinase fused to mTurquoise2 under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid

    #183411

    Purpose

    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    Tet-on 3G
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDH-CMV-mouse Gfi1-GFP

    Plasmid

    #91892

    Purpose

    3rd generation lentiviral expression of mouse Gfi1

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Gfi1 (Gfi1 Mouse)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    Unknown

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Kif5A-GFP-CIBN

    Plasmid

    #102252

    Purpose

    Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    Kif5A-GFP-CIB1 (Kif5a, CIB1 Mustard Weed, Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HsTPC2-EYFP

    Plasmid

    #135194

    Purpose

    Expresses tagged TPC2 in mammalian cells

    Depositor

    Antony Galione

    Article

    Davis et al Curr Biol. 2012 Dec 18;22(24):2331-7. do

    Insert

    TPC2 (TPCN2 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Strep-KIFC1*-mCh

    Plasmid

    #120169

    Purpose

    Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member C1 (KIFC1 Human)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIFC1: 125-673 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mCh-KIF5B*-strep

    Plasmid

    #120164

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5B (Kif5b Mouse)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF5B: 1-555 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LAMP1-SBP-GFP

    Plasmid

    #120172

    Purpose

    Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    Lysosomal-associated membrane protein 1 (Lamp1 Rat)
    

    Tags

    SBP and eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-FNLS-P2A-Puro

    Plasmid

    #110841

    Purpose

    Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CRY2-mCherry-Miro1TM

    Plasmid

    #102247

    Purpose

    Expresses fusion of CRY2PHR with mCherry and transmembrane domain of Miro1

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    CRY2PHR-mCherry-Miro1TM (RHOT1, CRY2 Mustard Weed, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. HA-KIF5B*-strep

    Plasmid

    #120170

    Purpose

    Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5B (Kif5b Mouse)
    

    Tags

    HA and Streptavidin

    Expression

    Mammalian

    Mutation

    Truncated KIF5B: 1-555

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TVBB N-term-mTurquoise2

    Plasmid

    #169222

    Purpose

    Targeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mTurquoise2
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TPC2(N257A)-mCherry

    Plasmid

    #135186

    Purpose

    Pore-dead mutant of TPC2, tagged on C terminus with mCherry.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Mutation of asparagine257 to alanine

    Available Since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TVBB C-term-mScarlet

    Plasmid

    #169219

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mScarlet at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. PEX3*-SBP-GFP

    Plasmid

    #120174

    Purpose

    Expresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    Peroxisomal biogenesis factor 3 (PEX3 Human)
    

    Tags

    SBP and eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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42. TPC2(E643A)-mCherry

    Plasmid

    #135187

    Purpose

    Mutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Mutation of glutamate 643 to alanine

    Available Since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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43. pmCherry N1 MFN2

    Plasmid

    #157759

    Purpose

    Expression of MFN2:mcherry in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    MFN2 (MFN2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA3_erGAP3

    Plasmid

    #78118

    Purpose

    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

    Depositor

    Javier García-Sancho, Teresa Alonso

    Article

    Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

    Insert

    erGAP3
    

    Tags

    KDEL and calreticulin signal peptide

    Expression

    Mammalian

    Mutation

    S175G, D180Y, I167V and L15Q in GFP moiety and D…

    Promoter

    CMV

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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45. pLenti-TRE3G-FNLS-PGK-Puro

    Plasmid

    #110847

    Purpose

    Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    TRE3G

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. Strep-KIFC1*-HA

    Plasmid

    #120171

    Purpose

    Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member C1 (KIFC1 Human)
    

    Tags

    HA and Streptavidin

    Expression

    Mammalian

    Mutation

    Truncated KIFC1: 125-673 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. mCh-KIF1A*-strep

    Plasmid

    #120165

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 1A (Kif1a Mouse)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF1A: 1-405 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. mCh-BICD2*-strep

    Plasmid

    #120168

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    BICD cargo adaptor 2 (Bicd2 Mouse)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated BICD2: 15-595 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. Kif5A-CIBN

    Plasmid

    #102251

    Purpose

    Expresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    Kif5A-CIB1 (Kif5a, CIB1 Mustard Weed, Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCDNA5/tom20-pbira(79-c)-ha

    Plasmid

    #154456

    Purpose

    expresses tom20-pbira(79-c)-ha/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    tom20-pbira(79-c)-ha (TOMM20 Human)
    

    Expression

    Mammalian

    Promoter

    CMV-F

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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