We narrowed to 22,183 results for: TES

1. pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

   Plasmid

   #83925

   Purpose

   Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   eGFP
   Bsr
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A

   Promoter

   hUbC

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBAD-yhjH

   Plasmid

   #111613

   Purpose

   Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

   Depositor

   Tim Tolker-Nielsen

   Article

   Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

   Insert

   yhjH
   

   Expression

   Bacterial

   Mutation

   A52V

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-IRES-tdTomato-FlagAkt1W80A

   Plasmid

   #64833

   Purpose

   cDNA of Akt1 with point mutation from Trp to Ala at position 80. This mutation confers resistance to the MK-2206 drug

   Depositor

   Eva Gonzalez

   Article

   Kajno et al Biochem J. 2015 Apr 9.

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Point mutation from Trp to Ala at position 80

   Promoter

   CMV

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

4. Biomolecular FeedBack (FB)

   Plasmid

   #109391

   Purpose

   This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Insert

   pJ23100mut-dCas9-T- phtpG1-sgRNA
   

   Expression

   Bacterial

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-p3E-HyPer7

   Plasmid

   #194289

   Purpose

   Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   Hyper7
   

   Use

   Multisite gateway entry clone

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Rab8WT(Flag) in pcDNA3.1neo

   Plasmid

   #46783

   Purpose

   expression of WT Rab8a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB8A (RAB8A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pTW072M

   Plasmid

   #115960

   Purpose

   Destination Vector

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl2 (nsP2 Q739L)
   

   Use

   Synthetic Biology

   Mutation

   nsP2 Q739L

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFlc245

   Plasmid

   #101021

   Purpose

   fluorescent, functional, glutamate receptor subunit

   Depositor

   Thomas Hughes

   Article

   Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

   Insert

   GluR 1 (Gria1 Rat)
   

   Expression

   Mammalian

   Mutation

   fluorescent protein inserted into subunit

   Promoter

   CMV

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

   Plasmid

   #162335

   Purpose

   Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   S. pyogenes sgRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZDonor-Nm-Cas9-gRNA-hU6

    Plasmid

    #61366

    Purpose

    empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTet-O-OVOL1-T2A-PuroR

    Plasmid

    #162347

    Purpose

    Lentiviral expression of OVOL1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    OVOL1 (OVOL1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Promoter

    Tet-ON

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. actc1b-mKate2-ING2

    Plasmid

    #109502

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-mKate2-ING2
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E-ML1N*2

    Plasmid

    #67678

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of ML1N*2

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-eYFP-FKBP-MAD1-WT

    Plasmid

    #114037

    Purpose

    Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

    Depositor

    Geert Kops

    Article

    Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

    Insert

    Human MAD1 (MAD1L1 Human)
    

    Tags

    eYFP-FKBP12

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

    Plasmid

    #67668

    Purpose

    Zebrafish expression vector for ubiquitous expression of APEX2-GBP

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Use

    Zebrafish expression with tol2

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3E-RhoAb-DN

    Plasmid

    #109580

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative RhoAb

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    RhoAb (rhoab Zebrafish)
    

    Use

    Zebrafish plasmids

    Mutation

    dominant negative

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-APEX2-P2A-mKate2

    Plasmid

    #67671

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-P2A-mKate2
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJB332

    Plasmid

    #115949

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 TetR-Gly9-DDX6 (contains SapI)
    

    Use

    Synthetic Biology

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTW122

    Plasmid

    #115962

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 5'-2xTetApt-mVenus-PEST
    

    Use

    Synthetic Biology

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAPcrRNA_Lmo

    Plasmid

    #126501

    Purpose

    L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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