We narrowed to 1,198 results for: e.coli

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pVRa28_1088

Plasmid

#49683

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF28_1088

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/hGGA3(GAT)

Plasmid

#79436

Purpose

Expresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coli

Depositor

Kazuhisa Nakayama

Article

Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

Insert

GGA3 (GGA3 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 107-286

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-GFP-PP-RPN2 (940-953)

Plasmid

#73746

Purpose

Expression of GFP-PP-RPN2 (940-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 940-953 of RPN2

Promoter

T7

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
Wild-type CoV-2 ORF8

Plasmid

#159933

Purpose

Protein overexpression in E.coli

Depositor

James Hurley

Article

Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

CoV-2 ORF8 18-121 (ORF8 CoV-2)

Tags

6x Histidine, TEV cleavable

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Halo-Tev-Keap1

Plasmid

#62456

Purpose

His6-halo tagged human Keap1 protein for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Bacterial

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pVRa42_4454

Plasmid

#49703

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF42_4454

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin 141-465

Plasmid

#45971

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 141-465 (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pTSSX-STC

Plasmid

#115668

Purpose

E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag

Depositor

David Kramer

Article

Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459

Insert

small tetraheme cytochrome c

Tags

Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…

Expression

Bacterial

Promoter

tac

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-848-952(pL91)

Plasmid

#124388

Purpose

E.Coli produced TET1-848-952 purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-848-952 wildtype (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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DT049A

Plasmid

#159410

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli tet promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tet-BDNF (Bdnf, Mouse) (Bdnf Synthetic, Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tet promoter

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/hGGA1(GAT)

Plasmid

#79435

Purpose

Expresses GST-tagged GAT domain of GGA1 (141-327 aa) in E.coli

Depositor

Kazuhisa Nakayama

Article

Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

Insert

GGA1 (GGA1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 141-327

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pVRa22_4450

Plasmid

#49676

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF22_4450

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa03_1198

Plasmid

#49655

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF03_1198

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCK389.AAV

Plasmid

#177327

Purpose

aTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expression

Depositor

James Carothers

Article

Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

Inserts

SoxS
dCas9

Use

CRISPR

Tags

MCP (MS2 Coat Protein)

Mutation

R93A,S101A

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin 77-465

Plasmid

#45970

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 77-465 (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-SF3B1-NTerm

Plasmid

#97425

Purpose

Expresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coli

Depositor

Alex Minella

Article

Murthy et al J Biol Chem. 2018 May 15. pii: RA118.001

Insert

SF3B1-N-term (AA1-500)

Tags

GST

Expression

Bacterial

Mutation

Silent mutation at nt876, aa289 (AGG to AGA)

Promoter

tac

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin 219-465

Plasmid

#45972

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 219-465 (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pVRc22_4450

Plasmid

#49741

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS22_4450

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin W403A-C431S

Plasmid

#45977

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin W403A-C431S (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Doub…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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J23101

Plasmid

#55176

Purpose

Very weak constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

J23101 promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

J23101

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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