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MiniCoopR 2xU6:gRNA pten, mitfa:Cas9

Plasmid

#118845

Purpose

Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

ptena and ptenb (ptena, ptenb Zebrafish)

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCS5-3HA/p14ARF

Plasmid

#78764

Purpose

To overexpress p14ARF in Mammalian Cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

p14ARF (CDKN2A Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV IE94

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-Nprot

Plasmid

#157728

Purpose

mammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nprot (N SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-GP-2-ATRX ADD

Plasmid

#59698

Purpose

Contains histone modification interacting domain (ATRX ADD)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

ATRX ADD (ATRX Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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Duffy-CD4d3+4-bio

Plasmid

#73122

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Duffy with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Duffy (ACKR1 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCcnb2

Plasmid

#162794

Purpose

Ccnb2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Ccnb2 shRNA (Ccnb2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCol1a1-frt (FVB)

Plasmid

#63575

Purpose

Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.

Depositor

Ivo Huijbers

Article

Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

Insert

Col1A-frt-hygro-pA

Use

Mouse Targeting; Frt/flpe

Expression

Bacterial and Mammalian

Promoter

None

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pSECC-sgTom

Plasmid

#138661

Purpose

Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgTomato

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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bio-CD4d3+4-Kell blood group

Plasmid

#73089

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Kell blood group with bio-rat CD4d3+4

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Kell blood group (KEL Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pECE HA AMPKa1 AS

Plasmid

#69831

Purpose

Expresses Expresses AMPK alpha 1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Tags

HA

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

Plasmid

#113681

Purpose

A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp12

Plasmid

#157710

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp12 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp14-HF

Plasmid

#157719

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp14-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSF4 TetCMV intron Renilla STOP 12xPP7 TNFα-ARE xrRNA12 24xMS2 SV40 CTE polyA

Plasmid

#104096

Purpose

Expresses TREAT-ARE reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Tags

12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

Expression

Mammalian

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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bio-CD4d3+4-TMEM24

Plasmid

#73092

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding TMEM24 with bio-rat CD4d3+4

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

TMEM24 (C2CD2L Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-P2A-CIAR_pLenti_CMV_Puro

Plasmid

#86503

Purpose

Lenti plasmid for generating CIAR expressing stable cell lines

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

GFP-P2A-CIAR (SOS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T968L in SOScat

Promoter

CMV

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF13_P2A_Hygro_Barcode

Plasmid

#120499

Purpose

Barcoded lentiviral vector to express KLF13 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF13 (KLF13 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp13-HF_GC3opt

Plasmid

#157716

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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LentiV_Neo_Sik3_T142Q

Plasmid

#119973

Purpose

Lentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

Sik3 (Sik3 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

change Threonine 142 to Glutamine

Promoter

EFS promoter

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBUN501

Plasmid

#50582

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

zCas9D10A
gRNA scaffold

Use

CRISPR; Plant expression

Tags

3xFLAG and NLS

Mutation

Cas9D10A nickase, was derived from the zCas9 and …

Promoter

AtU6-26p and Ubi1p

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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