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pTBL437 AAVS1 EQR sgRNA

Plasmid

#126447

Purpose

To cut the human AAVS1 locus for integration.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human AAVS1 safe-harbor locus with NGAG PAM

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

Plasmid

#181787

Purpose

Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ShTnsB, ShTnsC, ShCas12k-ShTniQ

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDule-IBBN (G2)

Plasmid

#85500

Purpose

Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

Insert

IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Expression

Bacterial

Mutation

Y32G L65E F108W Q109M D158S R162K

Promoter

lpp (constitutive)

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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NO- DSD Sensor 3

Plasmid

#199787

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO- DSD Sensor

Use

Synthetic Biology

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Tbxas1

Plasmid

#170305

Purpose

A knock-out vector for the mouse Tbxas1

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Tbxas1 gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-ER-EmEGFP

Plasmid

#169000

Purpose

Express ER-EmEGFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Synthetic, Human)

Use

Unspecified

Tags

EGFP

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKH162_FLAG-APEX2-YIPF3 LIR mut

Plasmid

#214981

Purpose

Flag-APEX2 tagged LIR mutant YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF3_mut (YIPF3 Human)

Use

Lentiviral

Mutation

LIR mutant

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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VQR variant

Plasmid

#138560

Purpose

Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VQR

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, T1337R

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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mEmerald-H2B-6

Plasmid

#54111

Purpose

Localization: Nucleus/Histones, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

H2B (H2BC11 Human)

Tags

mEmerald

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSR284

Plasmid

#125093

Purpose

Expression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-manR(DBD)122
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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SPLINTR BFP v1 backbone

Plasmid

#179779

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSL0373 (His-MBP-TniQ)

Plasmid

#130638

Purpose

Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on TniQ

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgMYC guide 2

Plasmid

#193597

Purpose

cMYC knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgMYC guide 2 (MYC Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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HA, EGFP Spastin M87 with exon 4, N386C

Plasmid

#167543

Purpose

For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4, with N386C mutation.

Depositor

Tarun Kapoor

Article

Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

Insert

Spastin M87 with exon 4 (SPAST Human)

Tags

EGFP and HA tag

Expression

Mammalian

Mutation

N386C

Promoter

CMV

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pKD279

Plasmid

#125099

Purpose

Expression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4388(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-evoA1-seBEN_empty

Plasmid

#174701

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

Lenti-evoA1-seBEN

Use

Lentiviral

Tags

myc tag

Promoter

EFS/U6

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSR241

Plasmid

#125091

Purpose

Expression of ompR(1-137aa)-yxdJ(132-229aa) chimera under PLtetO-1, output Promoter PyxdL181

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-yxdJ(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4

Plasmid

#123658

Purpose

DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.

Depositor

Stanley Qi

Article

Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

mU6

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSBK.013

Plasmid

#187213

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT])
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_OTmut

Plasmid

#185385

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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