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pET-22HT-MSI1(7-192)

Plasmid

#60245

Purpose

Express the RBD of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains aa7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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Dendra2-LC-Myosin-N-7

Plasmid

#57729

Purpose

Localization: Light Chain Myosin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Insert

LC-Myosin

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pONL-N1_zyxin

Plasmid

#65683

Purpose

Expresses Zyxin-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

zyxin (Zyx Mouse)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2015

Availability

Academic Institutions and Nonprofits only
jk65

Plasmid

#87019

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

SNAP-Nlg1AB
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Dronpa-SSTR3-N-17

Plasmid

#57296

Purpose

Localization: Cilium, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Insert

SSTR3

Tags

Dronpa

Expression

Mammalian

Mutation

A270V in SSTR3

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pBD-0005

Plasmid

#12281

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP K315L (Vcp Mouse)

Tags

His6

Expression

Bacterial

Mutation

K315L

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R61E

Plasmid

#60353

Purpose

R61E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R61E; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R53E/R59E/R61E

Plasmid

#60355

Purpose

R53E R59E R61E triple mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E, R59E and R61E mutations; contains amino aci…

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
Jam4.1-Fc-His

Plasmid

#72081

Purpose

Expresses the extracellular region of the JAM-4, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam4.1 (Igsf5 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse-MycNV419D-IRES-RFP

Plasmid

#73574

Purpose

Mammalian expression of mouse MycN V419D mutant

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

Mycn (Mycn Mouse)

Use

Retroviral

Tags

IRES-RFP

Expression

Mammalian

Mutation

V419D

Promoter

MSCV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pBD-0032

Plasmid

#12357

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP R362A (Vcp Mouse)

Tags

His6

Expression

Bacterial

Mutation

R362A

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only
Mouse c-myc (exons II and III)

Plasmid

#8448

Depositor

Frederic Mushinski

Article

Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

Insert

Mouse c-myc (exons II and III) (Myc Mouse)

Expression

Bacterial

Mutation

Exons II and III only

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
Neo1.c-AP-His

Plasmid

#71965

Purpose

Expresses the extracellular region of the Neogenin 1, isoform c protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.c (Neo1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Plxnb2(L)-AP-His

Plasmid

#72002

Purpose

Expresses the extracellular region of the PlexinB2 protein (ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb2 (Plxnb2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HA-mRIPK3 AAAA-Flag/cHA-pTRIPZ vector

Plasmid

#73702

Purpose

lentiviral expression of mRIPK3 mutant

Depositor

Francis Chan

Insert

RIPK3 (Ripk3 Mouse)

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

448-AAAA-451

Promoter

CMV/tet

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only
PBB-79

Plasmid

#8813

Depositor

Cynthia Wolberger

Article

Batchelor et al Science 1998 Feb 13;279(5353):1037-41.

Insert

GABPb1 (1-157) (Gabpb1 Mouse)

Expression

Bacterial

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO GFP DNAJB10

Plasmid

#19507

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB10 (DNAJB2 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Btg1

Plasmid

#45865

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Btg1 in situ probe (Btg1 Mouse)

Use

In situ

Mutation

contains bp# 1004-1638 of BC006834.1

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
mEmerald-Talin-Rod

Plasmid

#54270

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Talin-Rod

Tags

mEmerald

Expression

Mammalian

Mutation

Talin Rod Domain w/ K673N (variant CAA39588.1)

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBD-0028

Plasmid

#12365

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP D598N (Vcp Mouse)

Tags

His6

Expression

Bacterial

Mutation

D598N

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only