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pG-HIV-LRT

Plasmid

#104592

Purpose

Standard for qPCR

Depositor

Kristine Yoder

Publication

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

HIV-1 late reverse transcripts

Use

Cloning vector

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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sh-SRF

Plasmid

#100797

Purpose

expression of shRNA targeting SRF

Depositor

Joseph Gordon

Publication

Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

Insert

rat SRF

Use

RNAi

Promoter

H1

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pJW6.01

Plasmid

#62882

Purpose

Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

Depositor

Geraldine Seydoux

Publication

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

GFP::MEG-3

Tags

GFP

Expression

Worm

Promoter

meg-3

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-CLC

Plasmid

#193559

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR

Plasmid

#208379

Purpose

Episomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistance

Depositor

Daniel Hawiger

Publication

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Expression

Mammalian

Promoter

human CMV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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ZCCHC6

Plasmid

#156140

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ZCCHC6 (TUT7 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-mTmod3-WT

Plasmid

#70753

Purpose

mouse Tropomodulin3, WT for bacterial expression

Depositor

Weiping Han

Publication

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Tropomodulin3 (Tmod3 Mouse)

Tags

GST

Expression

Bacterial

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pRK-HA-TRAF4

Plasmid

#58314

Purpose

HA-tagged mouse TRAF4

Depositor

Ying Zhang

Publication

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3 N-MLSN

Plasmid

#21082

Depositor

Craig Montell

Publication

Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRPM1 (TRPM1 Human)

Tags

Myc

Expression

Mammalian

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pProEX-Af2Sir2

Plasmid

#8380

Depositor

Cynthia Wolberger

Publication

Avalos et al Mol Cell 2002 Sep;10(3):523-35.

Insert

Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)

Tags

His

Expression

Bacterial

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pColdI-SBP-eIF4A1

Plasmid

#233621

Purpose

Expression of SBP-tagged eIF4A1 in E.coli

Depositor

Shintaro Iwasaki

Publication

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

SBP-eIF4A1 (EIF4A1 Human)

Tags

His6-SBP

Expression

Bacterial

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-Cyclin B1

Plasmid

#215049

Purpose

Expresses EGFP-Cyclin B1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Publication

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCNB1 (CCNB1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

LTR

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pIA1363

Plasmid

#166861

Purpose

Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

Depositor

Irina Artsimovitch

Publication

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp8 (ORF1ab Synthetic)

Tags

His-GB1

Expression

Bacterial

Promoter

T7

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGWKS10

Plasmid

#139417

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.

Depositor

James Fraser

Publication

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

Clover

Expression

Yeast

Promoter

TEF1

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEB1-mEYFP

Plasmid

#103985

Purpose

Plasmid encoding mEYFP

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEYFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65G, V68L, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR221-YAP1 CA

Plasmid

#79502

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

YAP1 (YAP1 Human)

Use

Gateway Cloning

Tags

3XFLAG

Mutation

S127A

Promoter

no Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pVHed02

Plasmid

#82352

Purpose

Bxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmid

Depositor

Richard Murray

Publication

Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

Inserts

mKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites

Use

Synthetic Biology

Expression

Bacterial

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-SV40:GFP

Plasmid

#68483

Purpose

Retrovirus expressing GFP driven by SV40 promoter

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR and SV40

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pIK127

Plasmid

#65631

Purpose

Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

Depositor

Iskra Katic

Publication

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

Peft-3::gfp::h2b::tbb-2 3'UTR

Expression

Worm

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Gcn5(D608A)

Plasmid

#63707

Purpose

retroviral expression of enzyme-dead mouse Gcn5 in mammalian cells

Depositor

Kai Ge

Publication

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

Gcn5 (Kat2a Mouse)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

D608A mutation

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article