161,799 results

1. pSEVA451-Cpf1

   Plasmid

   #192362

   Purpose

   CRISPR-based vector for genome editing

   Depositor

   Juana María Navarro Llorens

   Article

   Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

   Insert

   Cas12a, gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-UCOE-SFFV-Zim3-dCas9-BFP

   Plasmid

   #188776

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9-mTagBFP2
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCE-BiFC-VC155

   Plasmid

   #22020

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Nat Protoc. 2008 . 3(4):588-96.

   Insert

   VC155
   

   Use

   Tags

   HA

   Expression

   Worm

   Mutation

   Changed Alanine 206 to Lysine

   Promoter

   Available since

   Nov. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIPZ-TIR1-HA

   Plasmid

   #172610

   Purpose

   Lentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   TIR1
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Tet/ON Minimal CMV promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmGFP11C-­Tau

   Plasmid

   #71434

   Purpose

   Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71433

   Depositor

   Henri Huttunen

   Article

   Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

   Insert

   Tau (0N4R) (MAPT Human)
   

   Use

   Tags

   GFP (213-228)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pScCas9

   Plasmid

   #117700

   Purpose

   Mammalian expression vector encoding WT ScCas9

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Sci Adv. 2018 Oct 24;4(10):eaau0766. doi

   Insert

   Streptococcus canis Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH-nCas9-PBE

   Plasmid

   #98163

   Purpose

   Expresses CRISPR base editor in rice

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Use

   Rice

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCLX-PGK-hCDK4

   Plasmid

   #114314

   Purpose

   Constitutive expression of the CDK4 protein

   Depositor

   Patrick Salmon

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-TIS11B

   Plasmid

   #121140

   Purpose

   Mammalian expression of mCherry tagged TIS11B

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   ZFP36L1 (TIS11B) (ZFP36L1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBE14

    Plasmid

    #174653

    Purpose

    In vivo Cre-lox circularization in E. coli

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    Cre
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-Puro (PX459)

    Plasmid

    #48139

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pRK5-HA-YFP-rictor

    Plasmid

    #73387

    Purpose

    Expresses HA and YFP tagged Rictor

    Depositor

    Taekjip Ha, Jie Chen

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)
    

    Use

    Tags

    HA and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB700 lenti gRNA Puro

    Plasmid

    #167911

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-AAV-sh[control]

    Plasmid

    #75438

    Purpose

    control shRNA vector

    Depositor

    Edward Burton

    Article

    Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

    Insert

    GFP
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid

    #171098

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    XbaI site added upstream of U6 promotor. GtoC mu…

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-eNme2-C-ABE8e

    Plasmid

    #185667

    Purpose

    Expresses eNme2-C-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEGFP-Lifeact-7

    Plasmid

    #54610

    Purpose

    Localization: Actin, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mEGFP-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV2.5-THP-GFP

    Plasmid

    #80336

    Purpose

    Fluorescent Reporter for Dopaminergic Neuron Differentiation

    Depositor

    Kwang-Soo Kim

    Article

    Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

    Insert

    rat TH promoter (Th Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Tyrosine hydroxylase

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. PBAD-his6-prkA-pACYC184

    Plasmid

    #41041

    Depositor

    Ichiro Matsumura

    Article

    Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

    Insert

    prkA
    

    Use

    Tags

    6xHis and T7 epitope tag

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAV-U6+27-Tornado-Pepper-RhoBAST

    Plasmid

    #196343

    Purpose

    Overexpression of circular Pepper-RhoBAST

    Depositor

    Murat Sunbul

    Article

    Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

    Insert

    Tornado-Pepper-RhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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