We narrowed to 12,738 results for: CAR

1. tRNA-gRNA position [2_n-1] (GB1206)

   Plasmid

   #75407

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   tRNA-gRNA position [2_n-1]
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3-HPRT1-S104R-PdTK-mCh

   Plasmid

   #107276

   Purpose

   HPRT1 c.312C>A Munich donor vector with unilateral microhomology

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Inserts

   HPRT genomic DNA (HPRT1 Human)
   puro-deltaTK; CAG::mCherry
   

   Use

   Tags

   Expression

   Mutation

   c.306G>T, c.312C>A

   Promoter

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.23 T230A 2014 EBOV + Mucin-Like Domain

   Plasmid

   #86017

   Purpose

   Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

   Depositor

   Pardis Sabeti, Jeremy Luban

   Article

   Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

   Insert

   Makona Ebola Virus Glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed threonine 230 to alanine

   Promoter

   CMV IE

   Available since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.23 D637G 2014 EBOV + Mucin-Like Domain

   Plasmid

   #86018

   Purpose

   Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

   Depositor

   Pardis Sabeti, Jeremy Luban

   Article

   Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

   Insert

   Makona Ebola Virus Glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed aspartic acid 637 to glycine

   Promoter

   CMV IE

   Available since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPN094

   Plasmid

   #91621

   Purpose

   Express sgRNA targeting human GRIN2A

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   GRIN2A (GRIN2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. His 3C Kinase dead MKK6 1-334

   Plasmid

   #87882

   Purpose

   E. coli expression of MKK6 kinase dead

   Depositor

   Peter Mace

   Article

   Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Tags

   3C protease site and 6xHis

   Expression

   Bacterial

   Mutation

   K82A, kinase dead mutant

   Promoter

   T7

   Available since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Frt-V5-hspB3

   Plasmid

   #63104

   Purpose

   Expression of HSPB3 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb3 (HSPB3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. RIPK2 gRNA (BRDN0001148477)

   Plasmid

   #76911

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RIPK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RIPK2 (RIPK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGB3 alpha1 35s:PIF6:tNos (GB0170)

   Plasmid

   #160606

   Purpose

   TU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

   Insert

   PIF6
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. tRNA-gRNA position [D1_2] (GB1205)

    Plasmid

    #75406

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    tRNA-gRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-mCherry-p2A-LIC1 G domain

    Plasmid

    #74602

    Purpose

    expresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherry

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGB 35s:dCas:VP64:tNos (GB1189)

    Plasmid

    #75403

    Purpose

    Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional Activator

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    dCas:VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSico shMAPKAPK3 human

    Plasmid

    #85661

    Purpose

    expression of shRNA targeting human MAPKAPK3

    Depositor

    Julien Sage

    Article

    Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

    Insert

    MAPKAPK3 (MAPKAPK3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMyc-CMV1-huTLR4mut-C2141A

    Plasmid

    #53527

    Purpose

    expression of mutant TLR4 in mammalian cells

    Depositor

    Linda Yu

    Article

    Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

    Insert

    TLR4 C2141A (TLR4 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C2141A

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6_sgRNA_CAG_hSt1Cas9_CNRZ1066

    Plasmid

    #136651

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9:CNRZ_1066 Chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and hU6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p-super-retro puro RhoGDI2 shRNA

    Plasmid

    #58897

    Purpose

    produces an shRNA against RhoGDI2

    Depositor

    Channing Der

    Article

    Stevens et al Small GTPases. 2011 Jul;2(4):202-210. Ep

    Insert

    RhoGDI2 (ARHGDIB Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pTAMAHISTEV_PfeAR480A-Q482A

    Plasmid

    #128946

    Purpose

    expresses R480A Q482A mutant of PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75), …

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTBL649 CHYRON2 integration construct

    Plasmid

    #126446

    Purpose

    To integrate the CHYRON2 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON2 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL4.23 A82V/T230A 2014 EBOV + Mucin-Like Domain

    Plasmid

    #86019

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Pardis Sabeti, Jeremy Luban

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed alanine 82 to valine and threonine 230 to…

    Promoter

    CMV IE

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3-HPRT1-S104Rf-PdTK-mCh

    Plasmid

    #107277

    Purpose

    HPRT1 c.312C>A (Munich) donor vector with bilateral microhomology

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Inserts

    HPRT genomic DNA (HPRT1 Human)
    puro-deltaTK; CAG::mCherry
    

    Use

    Tags

    Expression

    Mutation

    c.306G>T, c.312C>A

    Promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge