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hFLN29-Mid/pCMV-14-FLAG (L175-D390)

Plasmid

#73589

Purpose

Express middle part (L175-D390) of human TRAFD1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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hFLN29-N/pCMV-14-FLAG (1-I210)

Plasmid

#73588

Purpose

Express N-terminal part (1-I210) of hTRAFD1 (FLN29) in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET23a-MmUBB(SnaBI)

Plasmid

#69558

Purpose

In vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Mutation

The two last codons (TATTAA; encoding the last ty…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET23a-MmUBB(Y305P)

Plasmid

#69557

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Mutation

Codon TAT encoding tyrosine 305 was changed to CC…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)

Plasmid

#68213

Purpose

Transcriptional unit for Hygromycin resistance gene plant expression

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hyg

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMyb12 (GB0075)

Plasmid

#68199

Purpose

Provides the CDS of theSolanum lycopersicum Myb12 transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Myb12 coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pT2A11 (GB0143)

Plasmid

#68189

Purpose

Provides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pTE8 (GB0144)

Plasmid

#68190

Purpose

Provides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlE8 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLexAOp (GB0118)

Plasmid

#68181

Purpose

Provides the 4xLexA Operator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD_Mini35s(-60,UTR) (GB0050)

Plasmid

#68176

Purpose

Provides the CaMV Mini35S Promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

CaMV Mini35S promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pIR195

Plasmid

#64810

Purpose

TCP4 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

TCP4 (TCP4 Mustard Weed)

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pIR196

Plasmid

#64811

Purpose

SPL9 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

SPL9 (SPL9 Mustard Weed)

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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ROZA YF

Plasmid

#64197

Purpose

Fluorescent Reporter for ZAP-70 tyrosine kinase activity-control YF mutant

Depositor

Annemarie Lellouch

Article

Randriamampita et al PLoS One. 2008 Jan 30;3(1):e1521. doi: 1

Insert

ROZA YF

Expression

Mammalian

Mutation

tyrosine phosphorylatin site mutated to phenylala…

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pSicoR-ADAM21i1

Plasmid

#59604

Purpose

Expression of shRNA against human ADAM21

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM21 (ADAM21 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-SLC17A5i1

Plasmid

#59598

Purpose

Expression of shRNA against human SLC17A5

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

SLC17A5 (SLC17A5 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-MannII-N-10

Plasmid

#55497

Purpose

Localization: Mannosidase/Golgi, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

MannII

Tags

mTFP1

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-SSTR3-N-17

Plasmid

#55509

Purpose

Localization: Cilium, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

SSTR3

Tags

mTFP1

Expression

Mammalian

Mutation

A270V in SSTR3

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-MCHR1-N-10

Plasmid

#55499

Purpose

Localization: Cilium, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

MCHR1

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-PTPRJi2

Plasmid

#59609

Purpose

Expression of shRNA against human PTPRJ

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPRJ (PTPRJ Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-SLC17A5i2

Plasmid

#59599

Purpose

Expression of shRNA against human SLC17A5

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

SLC17A5 (SLC17A5 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only