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HT-mGold

Plasmid

#183986

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells in the whole cell.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

HaloTag-mGold

Tags

HaloTag, mGold

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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LT2_12_GlyRNA

Plasmid

#177710

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Hygro

Plasmid

#175267

Purpose

MegaGate destination with C-terminal DD tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

Destabilization Domain

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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EZ-L545

Plasmid

#173024

Purpose

Encodes OptoEXP2 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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EZ-L580

Plasmid

#173023

Purpose

Encodes OptoAMP4 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCS4083

Plasmid

#131739

Purpose

Two Color Control Assay bicistronic BFP-mCherry-sTRSVctl

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

sTRSV

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 delta N (aa236-314) BLAST

Plasmid

#164235

Purpose

lentiviral plasmid for GFP-TREX1 delta N (aa236-314)

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deletion of amino acids 1-234

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pGL001

Plasmid

#115674

Purpose

Fluorescent reporter for E.coli K12 fliC promoter activity

Depositor

Gene-Wei Li

Article

Parker et al J Bacteriol. 2019 Sep 6;201(19). pii: JB

Insert

fliC promoter fused with GFP

Tags

Green fluorescent protein (GFP)

Expression

Bacterial

Promoter

fliC promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV2-N587X

Plasmid

#130877

Purpose

Encodes the AAV2 capsid with the N587 residue replaces with a TAG stop codon

Depositor

Prashant Mali

Article

Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

Insert

AAV2 cap N587X

Use

AAV

Expression

Mammalian

Mutation

N587X

Promoter

Unknown

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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PC4_3spc34h

Plasmid

#70700

Purpose

Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on spc34

Depositor

Stephen Harrison

Article

Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

Insert

DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)

Tags

his-tag on C-terminus of spc34

Expression

Bacterial

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-rsTagRFP

Plasmid

#87620

Purpose

Endoplasmic reticulum (ER)-targeted rsTagRFP.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

ER-rsTagRFP

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHES822

Plasmid

#89193

Purpose

Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Expression

Yeast

Promoter

pZ

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-pro-alphaf-E2-Crimson

Plasmid

#117658

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-pro-alphaf-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMRXIP-Myc-SNAP29

Plasmid

#116944

Purpose

Expressing Myc-SNAP29 for mammalian cells

Depositor

Noboru Mizushima

Article

Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

Insert

synaptosomal-associated protein, 29kDa (SNAP29 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-2-84-FLAG

Plasmid

#35980

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV2 84 amino acids

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pQZ083

Plasmid

#106417

Purpose

Express Complexin-1 fragment (1-83) in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Insert

complexin-1 fragment 1-83 (Cplx1 Rat, Synthetic)

Tags

GST tag

Expression

Bacterial

Mutation

Fragment 1-83, Codon optimization

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDC183

Plasmid

#106415

Purpose

Co-expresses trans SNARE complex with pQZ210 in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Inserts

SNAP25 fragment 141-204 (Snap25 Rat, Synthetic)
SNAP25 Fragment 7-83 (Snap25 Rat, Synthetic)

Expression

Bacterial

Mutation

Fragment 141-204, Codon optimization and Fragment…

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Adrb2

Plasmid

#105199

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Adrb2 (Adrb2 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-nlsGFP no-pA

Plasmid

#80815

Purpose

nlsGFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-CMV-nlsGFP no pA

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-GFPmem no-pA

Plasmid

#80817

Purpose

GFPmem without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFPcaax no pA p3E

Expression

Mammalian

Promoter

GFP

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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