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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,068 results for: transfer

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Showing: 8301 - 8320 of 10068 results

  12. CSTI.pJL1

    Plasmid
    #199113
    Purpose
    In vitro expression of glycosyltransferase from Campylobacter jejuni for elaboration of sialic acid onto galactose in α2,3 linkage using CMP-sialic acid as substrate
    Depositor
    Michael Jewett
    Article
    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102
    Insert
    CSTI
    Tags
    CAT linker, Strep-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199113-plasmid-map-sequence-id-390798

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  13. pSilencer2.1-U6-T1-U6-bc339

    Plasmid
    #190849
    Purpose
    For mammalian expression of T1 and bc339, the RNA aptamers of ncOGT and β-catenin, respectively. Expression of each aptamer is driven by a U6 promoter.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Inserts
    T1 (an RNA aptamer of O-GlcNAc Transferase)
    bc339 (an RNA aptamer of β-catenin)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190849-plasmid-map-sequence-id-379246

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  20. pTJV1ScGm-rpoB

    Plasmid
    #166979
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aacC1 for gentamycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    gentamycin-3-acetyltransferase (aac(3)-Ia )
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166979-plasmid-map-sequence-id-331330

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Showing: 8301 - 8320 of 10068 results