2,294 results

1. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

   Plasmid

   #218864

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8s
   

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   S26M F286Y Q315H

   Promoter

   hsp70

   Available Since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-SYN_mCherry-KIX-mCherry

   Plasmid

   #137007

   Purpose

   AAV backbone, mCherry-KIX acceptor for G-CREB

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

   Insert

   KIX domain (Crebbp Rat)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-HA-ERK2 D319N

   Plasmid

   #8979

   Depositor

   John Blenis

   Article

   Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

   Insert

   ERK2 (Mapk1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D319N

   Available Since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pGAL4-CREB S133A

   Plasmid

   #15222

   Depositor

   Ugo Moens

   Article

   Delghandi et al Cell Signal. 2005 Nov . 17(11):1343-51.

   Insert

   CREB S133A (Creb1 Rat)
   

   Tags

   Gal4

   Expression

   Mammalian

   Mutation

   Serine 133 mutated to Alanine

   Available Since

   June 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

   Plasmid

   #68719

   Purpose

   Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

   Depositor

   Tobias Bonhoeffer, Mark Huebener, Tobias Rose

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6f
   

   Use

   AAV

   Promoter

   CAG-FLEX

   Available Since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.hSyn.FLEX.iGluSnFR3.v857.IgK-NGR

   Plasmid

   #196226

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV hSyn FLEX iGluSnFR3 v857.NGR
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and NGR TM Domain

   Expression

   Mammalian

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM60-S6K1(wt) barcoded

   Plasmid

   #48801

   Purpose

   expresses wt S6K1

   Depositor

   David Sabatini

   Article

   Kang et al Science. 2013 Jul 26;341(6144):1236566.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG and barcode: GGATCC

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. p3xFlag-CMV7-Erk2_KR

   Plasmid

   #39224

   Depositor

   Melanie Cobb

   Article

   Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

   Insert

   Erk2(K52R) (Mapk1 Rat)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K52R

   Promoter

   CMV

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

9. mTagRFP-T-Cx26-7

   Plasmid

   #58007

   Purpose

   Localization: Gap Junctions, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Cx26 (Gjb2 Rat)
   

   Tags

   mTagRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. C1-FT-mCitrine-EEVEE-CaaX

    Plasmid

    #155225

    Purpose

    MPAct with increased linker flexiblity

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b
    

    Tags

    mCitrine

    Expression

    Mammalian

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Halo-p58

    Plasmid

    #166896

    Purpose

    mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    p58 (Lman1 Rat)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    L405Q, R419S- please see depositor comments

    Promoter

    CMV

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. BE3-P2A-EGFP (pJUL977)

    Plasmid

    #123612

    Purpose

    CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3-P2A-EGFP
    

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CAG

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSIN REP5-GFP-GluR2 (Q)

    Plasmid

    #24003

    Depositor

    Robert Malinow

    Article

    Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

    Insert

    GluR2 (Gria2 Rat)
    

    Use

    Sindbis virus production

    Tags

    GFP

    Mutation

    R607Q

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-PSD95-GCaMP2

    Plasmid

    #18931

    Depositor

    Karel Svoboda

    Article

    Mao et al PLoS ONE. 2008 . 3(3):e1796.

    Insert

    PSD95-GCaMP2 (Dlg4 Rat)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

15. E. coli expression: Z-lock cofilin dark state mutant

    Plasmid

    #141135

    Purpose

    a control for optogenetic cofilin analog

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Expression

    Bacterial

    Mutation

    D3 deletion, I32F in Zdk2

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-CMV-Rheb(D60K)-IRES-eGFP-W

    Plasmid

    #32522

    Depositor

    Mustafa Sahin

    Insert

    dominant negative Rheb (D60K) (Rheb Rat)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    D60K to make dominant negative

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

17. AAV-Syn-H2B-jGCaMP8m-WPRE

    Plasmid

    #176748

    Purpose

    AAV-mediated expression of histone fused GCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    H2B-jGCaMP8m
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    synapsin

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dynamin-I-K44A-6His/Flag

    Plasmid

    #32696

    Depositor

    Robert Lefkowitz

    Article

    Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

    Insert

    Dynamin I (Dnm1 Rat)
    

    Tags

    Flag and His6

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    CMV

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

19. mCerulean3-Lysosomes-20

    Plasmid

    #55428

    Purpose

    Localization: Lysosome Membrane, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    LAMP1
    

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. BE3-Gam

    Plasmid

    #100807

    Purpose

    Expresses BE3-Gam in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

    Insert

    BE3-Gam
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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