2,339 results

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pMK279 (mClover-Bsr)

Plasmid

#72795

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Bsr

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-IP GFP-Omp25

Plasmid

#38249

Depositor

Noboru Mizushima

Article

Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

Insert

Omp25 (Synj2bp Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

plasmid contains AA109-145 of rat Omp25/Synj2bp

Available Since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only

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cacnb4 (rat) in pMT2 vector

Plasmid

#107426

Purpose

Expresses Calcium channel beta4 auxillary subunit in mammalian cells

Depositor

Annette Dolphin

Article

Brodbeck et al J Biol Chem. 2002 Mar 8;277(10):7684-93.

Insert

cacnb4 (Cacnb4 Rat)

Expression

Mammalian

Promoter

Ad MLP

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Wnk1

Plasmid

#38780

Depositor

Melanie Cobb

Article

Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

Insert

Wnk1 (Wnk1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pFSW Syt1 WT IRES GFP

Plasmid

#133816

Purpose

Encodes full-length wild type synaptotagmin 1 for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Promoter

hSyn (human synapsin I promoter)

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-rnSOX9

Plasmid

#62972

Purpose

Expresses HA-tagged rat SOX9

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Tags

HA

Expression

Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
EGFP-Septin 11 isoform II

Plasmid

#120317

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the N-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-CaMKIIbeta(K43R)

Plasmid

#21225

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CaMKIIbeta(K43R) (Camk2b Rat)

Tags

GFP

Expression

Mammalian

Mutation

CaMKIIbeta(K43R) Lab plasmid CF 0003.

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK2 D319N

Plasmid

#8979

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

D319N

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
mEmerald-Homer1-N-18

Plasmid

#54121

Purpose

Localization: Membrane Adaptor, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Homer1 (Homer1 Rat)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Rescue Septin 11 mRNA

Plasmid

#122022

Purpose

Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Mutation

Silent mutations are in 5 consecutive codons (AUU…

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Kaede-Lysosomes-20

Plasmid

#57318

Purpose

Localization: Lysosome Membrane, Excitation: 508 / 572, Emission: 518 / 580

Depositor

Michael Davidson

Insert

LAMP1

Tags

Kaede

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pMRXIP Lamp1-Venus

Plasmid

#89937

Purpose

Expresses Lamp1 tagged with Venus in mammalian cells

Depositor

Noboru Mizushima

Article

Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

Insert

rat Lamp1 (Lamp1 Rat)

Use

Retroviral

Tags

Venus

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-2xmCherry-EF(C)

Plasmid

#61664

Purpose

Tandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

Plasmid

#68719

Purpose

Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Has Service

AAV1

Insert

mRuby2-P2A-GCaMP6f

Use

AAV

Promoter

CAG-FLEX

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PSD95-GCaMP2

Plasmid

#18931

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

PSD95-GCaMP2 (Dlg4 Rat)

Tags

His

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pGAL4-CREB S133A

Plasmid

#15222

Depositor

Ugo Moens

Article

Delghandi et al Cell Signal. 2005 Nov . 17(11):1343-51.

Insert

CREB S133A (Creb1 Rat)

Tags

Gal4

Expression

Mammalian

Mutation

Serine 133 mutated to Alanine

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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pEF HaloTag-SYB2

Plasmid

#197282

Purpose

Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYB2 and HaloTag (Vamp2 Rat)

Expression

Mammalian

Mutation

Q76V and F77W

Promoter

EF1alpha

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFlag-CMV7-Erk2_KR

Plasmid

#39224

Depositor

Melanie Cobb

Article

Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

Insert

Erk2(K52R) (Mapk1 Rat)

Tags

3xFLAG

Expression

Mammalian

Mutation

K52R

Promoter

CMV

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pGEX Syt1 C2AB (96-421)

Plasmid

#170643

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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