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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3,272 results for: aaas

Showing: 1641 - 1680 of 3272 results

  7. pCF806-shABC2

    Plasmid
    #186714
    Purpose
    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.
    Depositor
    Christof Fellmann
    Article
    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023
    Insert
    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    186714-plasmid-map-sequence-id-382420

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  8. pCF806-shABC1

    Plasmid
    #186713
    Purpose
    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.
    Depositor
    Christof Fellmann
    Article
    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023
    Insert
    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    186713-plasmid-map-sequence-id-376087

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  9. hNTo1-qgRNA-pYJA5

    Plasmid
    #217781
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217781-plasmid-map-sequence-id-466019

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  18. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh1-guide3

    Plasmid
    #125402
    Purpose
    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    125402-plasmid-map-sequence-id-251102

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  20. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide3

    Plasmid
    #118179
    Purpose
    CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118179-plasmid-map-sequence-id-240448

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  22. pCGLH-luciferase

    Plasmid
    #194979
    Purpose
    pCGLH control plasmid
    Depositor
    Angelique Bordey
    Insert
    luciferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    194979-plasmid-map-sequence-id-386135

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  23. pSico-shHDAC2

    Plasmid
    #194983
    Purpose
    conditional knock down HDAC2
    Depositor
    Angelique Bordey
    Insert
    shHDAC2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    194983-plasmid-map-sequence-id-386019

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  24. SGEP-shRLuc-802

    Plasmid
    #188668
    Purpose
    control shRNA
    Depositor
    Lewis Cantley
    Insert
    shRLuc
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188668-plasmid-map-sequence-id-375059

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  25. ipUSEPR-sg-Hs-PCNA

    Plasmid
    #188686
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPCNA (PCNA Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188686-plasmid-map-sequence-id-375116

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  26. T4 Lysozyme N53A N55A V57A

    Plasmid
    #18353
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N53A N55A V57A
    Promoter
    Available since
    Aug. 26, 2008
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV CAG Barcode6

    Plasmid
    #229068
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    CAG Barcode6
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    229068-plasmid-map-sequence-id-455091

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  28. CMV-hEAAT5

    Plasmid
    #32817
    Depositor
    Susan Amara
    Insert
    EAAT5 (SLC1A7 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2012
    Availability
    Academic Institutions and Nonprofits only

  29. pAAV syn Barcode1

    Plasmid
    #229062
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode1
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    229062-plasmid-map-sequence-id-454825

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  30. pAAV Syn Barcode11

    Plasmid
    #226185
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode11
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    226185-plasmid-map-sequence-id-447874

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  31. mKO2-CytERM-N-17

    Plasmid
    #57868
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 551, Emission: 565
    Depositor
    Michael Davidson
    Insert
    CytERM
    Use
    Tags
    mKO2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  32. pBACH2.2.0-gDNA

    Plasmid
    #176375
    Purpose
    Expresses spCas9 and gRNA sequence targeting BACH2
    Depositor
    Kevin White, Barbara Stranger
    Insert
    BACH2 gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    176375-plasmid-map-sequence-id-347302

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  33. pDECKO_GFP_AAVS1_186

    Plasmid
    #159091
    Purpose
    targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    159091-plasmid-map-sequence-id-308784

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  34. pDECKO_mCherry_AAVS1

    Plasmid
    #141212
    Purpose
    pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    141212-plasmid-map-sequence-id-299605

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  35. pPOU6F1.1.0-gDNA

    Plasmid
    #176377
    Purpose
    Expresses spCas9 and gRNA sequence targeting POU6F1
    Depositor
    Kevin White, Barbara Stranger
    Insert
    POU6F1 gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176377-plasmid-map-sequence-id-347371

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  36. mApple-MAP4-C-10

    Plasmid
    #54923
    Purpose
    Localization: Microtubules, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    MAP4
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    aa 640-1045 of MAP4
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pREP4xN_ccdB

    Plasmid
    #79022
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79022-plasmid-map-sequence-id-147779

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  38. C33m

    Plasmid
    #120968
    Purpose
    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AiiA degradase (Bennett, no ssra tag)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120968-plasmid-map-sequence-id-238044

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  39. pREP3xN_ccdB

    Plasmid
    #79021
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79021-plasmid-map-sequence-id-151965

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  40. LT3GEPIR-NEK7shRNA2

    Plasmid
    #220551
    Purpose
    To inducibly knockdown NEK7 expression
    Depositor
    Sean Lee
    Insert
    NEK7 shRNA2 (NEK7 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220551-plasmid-map-sequence-id-437960

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Showing: 1641 - 1680 of 3272 results