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pTrc-clbS_Ealbertii

Plasmid

#223360

Purpose

Encoding ClbS from Escherichia albertii CB9786

Depositor

Emily Balskus

Article

Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

Insert

Encoding ClbS from Escherichia albertii CB9786 (Codon optimized for E coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTrc

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ptRNAfMet-A37U

Plasmid

#196659

Purpose

Plasmid for overexpression of mutated initiator tRNA (A37U)

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

tRNAfMet-A37U expression cassette

Use

Tags

Expression

Bacterial

Mutation

A37U in tRNAfMet

Promoter

metY native promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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ptRNAfMet-A37G

Plasmid

#196658

Purpose

Plasmid for overexpression of mutated initiator tRNA (A37G)

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

tRNAfMet-A37G expression cassette

Use

Tags

Expression

Bacterial

Mutation

A37G in tRNAfMet

Promoter

metY native promoter

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Cep41-STf

Plasmid

#182010

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines associated to microtubules

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Cep41-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_[Cox8a]x2_STf

Plasmid

#182003

Purpose

CMV overexpression of SNAP-tag fast in the mitochondrial matrix of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

[Cox8a]x2_STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR_STf_KDEL

Plasmid

#182004

Purpose

CMV overexpression of SNAP-tag fast in the endoplasmic reticulum of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

CalR_STf_KDEL

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_b4g-STf

Plasmid

#182005

Purpose

CMV overexpression of SNAP-tag fast in the golgi apparatus of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

b4g-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pNCS-Clover4-LUMABS-HA

Plasmid

#183486

Purpose

To express the Clover4-LUMABS-HA sensor for Avian Influenza Virus antibody detection.

Depositor

Jun Chu, Zongwen Jin

Article

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-HA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pNCS-Clover4-LUMABS-DEN1

Plasmid

#183487

Purpose

To express the Clover4-LUMABS-DEN1 sensor for Dengue Virus antibody dection.

Depositor

Jun Chu, Zongwen Jin

Article

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-DEN1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140170

Purpose

Expresses a regulatable secretory cargo for Arabidopsis thaliana

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140169

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thaliana

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Plasmid

#140155

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.9

Plasmid

#78179

Purpose

Expresses-rsGreen0.9 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.9

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.7b

Plasmid

#78178

Purpose

Expresses rsGreen0.7b in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.7b

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pfMDH_Triple

Plasmid

#67733

Purpose

Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Use

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed histidine 303 to alanine, arginine 373 to…

Promoter

T7

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pNB812

Plasmid

#60160

Purpose

Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

yEVenus-PEST

Use

Tags

PEST degron

Expression

Yeast

Mutation

Promoter

RNR1pr

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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mTFP1-MannII-N-10

Plasmid

#55497

Purpose

Localization: Mannosidase/Golgi, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

MannII

Use

Tags

mTFP1

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-SSTR3-N-17

Plasmid

#55509

Purpose

Localization: Cilium, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

SSTR3

Use

Tags

mTFP1

Expression

Mammalian

Mutation

A270V in SSTR3

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-MCHR1-N-10

Plasmid

#55499

Purpose

Localization: Cilium, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

MCHR1

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pANAP

Plasmid

#48696

Purpose

Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

Depositor

Peter Schultz

Article

Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

Inserts

8 copies of the amber suppressor tRNACUA-Leu
AnapRS

Use

Tags

Expression

Mammalian

Mutation

mutant E.coli leucyl synthetase specific for Anap…

Promoter

CMV and human H1 promoter

Available since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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