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Plasmid#216385PurposeBaculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)DepositorInsertNorrin (NDP Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3C and Rho1D4ExpressionInsectMutationC93A/C95A/C131APromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-SNAP
Plasmid#206480PurposeEncodes a mitochondrially-targeted SNAP tag for mammalian cell expressionDepositorInsertMitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
UseTagsSNAPfExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAB18::LUC
Plasmid#141168PurposeLuminescent reporter for abscisic acid signalingDepositorInsertAtRAB18 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtRAB18, AT5G66400Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKar2-msGFP-HDEL
Plasmid#117668PurposeLocalisation of Endoplasmic ReticulumDepositorInsertpKar2-msGFP-HDEL
UseTagsExpressionYeastMutationContains the Kar2 promoter and signal sequence fr…PromoterpKar2Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLX-M38s/TOP-dGFP
Plasmid#114295PurposeExpression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoterDepositorInsertdGFP
UseLentiviralTagsExpressionMutationdestabilized GFPPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIAA5::LUC
Plasmid#141169PurposeLuminescent reporter for auxin signalingDepositorInsertAtIAA5 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtIAA5, At1g15580Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Bov.hsc70.RSET.FL.wt(NarI)
Plasmid#12532DepositorInsertbovine 70 kDa heat shock cognate protein full length wild type
UseTagsExpressionBacterialMutationwild typePromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWKS1583
Plasmid#70188PurposeModular vector for secreted amylase reporter expressionDepositorInsertamyEopt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta4
Plasmid#101292PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation76-1354 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta6
Plasmid#101294PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation375-1354 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAL189
Plasmid#22278PurposeExpresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitmentDepositorInsertIntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKH-MoHyPer
Plasmid#78267PurposeExpress HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.DepositorInsertMoHyper
UseExpress in magnaportheTagsExpressionYeastMutationPromoterRp27Available sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R739A
Plasmid#84933PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.DepositorInsertcAMPr Y205A R739A
UseTagsExpressionInsectMutationPKA-C Y205A, PKA-R R739APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R626A
Plasmid#84932PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.DepositorInsertcAMPr Y205A R626A
UseTagsExpressionInsectMutationPKA-C Y205A, PKA-R R626APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta7
Plasmid#101295PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation727-1354 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-rhodopsin-CTS
Plasmid#86071PurposeA bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.DepositorInsertRhodopsin (RHO Bovine)
UseTagsGSTExpressionBacterialMutationcontains the region from 309-348 of RhodopsinPromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV0764
Plasmid#133520PurposeExpression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0773
Plasmid#133519PurposeExpression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAZ10::LUC
Plasmid#141172PurposeLuminescent reporter for jasmonic acid signalingDepositorInsertAtJAZ10 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtJAZ10, AT5G13220Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only