We narrowed to 24,588 results for: Nes

1. pETM33_SIN3A_PAH

   Plasmid

   #178486

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   SIN3A_PAH (SIN3A Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

   Plasmid

   #223174

   Purpose

   Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   RfxCas13d
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mutation

   Promoter

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB-CAG-TEVp-IRES-mCherry

   Plasmid

   #174377

   Purpose

   For making TEVP cell line with hypBase

   Depositor

   Ian Wickersham

   Article

   Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

   Insert

   TEV Protease
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDN34

   Plasmid

   #61343

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   GS linker-AsLov2-biNLS2 and PKIt NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-TREtight-EGFP

   Plasmid

   #182967

   Purpose

   For making cell lines for titering pRV∆L-5tTA virus

   Depositor

   Ian Wickersham

   Article

   Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   EGFP
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRV∆L-5Cre

   Plasmid

   #182964

   Purpose

   The 3rd generation of rabies vector expressing Cre recombinase

   Depositor

   Ian Wickersham

   Article

   Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   CRE recombinase
   

   Use

   Rabies

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGI3EM22C

   Plasmid

   #135488

   Purpose

   Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr LifeAct-tdTomato
   

   Use

   Tags

   LifeAct

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2A/Bpr (divergent)

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-U-TVA950

   Plasmid

   #115236

   Purpose

   Lentiviral transfer plasmid for expression of TVA950

   Depositor

   Ian Wickersham

   Article

   Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

   Insert

   TVA950
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB22

   Plasmid

   #61342

   Purpose

   Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   GS linker-AsLov2-cMyc1A NLS and PKIt NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGI3EM20C

    Plasmid

    #135487

    Purpose

    Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr H2B-tdTomato
    

    Use

    Tags

    tdTomato

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSimpleII-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47867

    Purpose

    This plasmid encode NmCas9 with two NLS and a HA tag

    Depositor

    James Thomson, Erik Sontheimer

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Insert

    NmCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. EcPPK1

    Plasmid

    #108850

    Purpose

    Expresses E. coli PPK1 in mammalian cell lines

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Polyphosphate Kinase 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM33_PEX14_Pex14

    Plasmid

    #178484

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    PEX14_Pex14 (PEX14 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETM33_OXSR1_CCT

    Plasmid

    #178482

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    OXSR1_OXSR1 (OXSR1 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNB785

    Plasmid

    #60164

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNB787

    Plasmid

    #60152

    Purpose

    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNB789

    Plasmid

    #60166

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    RNR1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Human Mevalonate diphosphate decarboxylase (MDD)

    Plasmid

    #52431

    Purpose

    bacterial expression of human Mevalonate diphosphate decarboxylase (MDD)

    Depositor

    Henry Miziorko

    Article

    Voynova et al Arch Biochem Biophys. 2008 Dec 1;480(1):

    Insert

    mevalonate diphosphate decarboxylase (MVD Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pLentiRNACRISPR_010-TetO-NLS-RfxCas13d-2A-mCherry-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #228555

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

    Insert

    RfxCas13d
    

    Use

    Lentiviral

    Tags

    NLS-HA and P2A-mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiRNACRISPR_011-TetO-NLS-inactive-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #228557

    Purpose

    Expresses inactive RfxCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

    Insert

    dRfxCas13d
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    R239A/H244A/R858A/H863A

    Promoter

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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