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Plasmid#91797PurposeLentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG systemDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsFKBP F36VExpressionMammalianMutationPromoterhPGKAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only
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pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB
Plasmid#193719PurposeRapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transportDepositorInserthsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Human, Synthetic)
UseTags2xHA-FRBExpressionMammalianMutationhsKIF5B(aa1-560), RIGOR: G234APromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre2_60.F2
Plasmid#60210PurposeExpresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)DepositorInsertCre recombinase (CD207 Human, Synthetic, Bacteriophage P1)
UseTagsFKBP12(F36V) and Nuclear localisation signalExpressionMammalianMutationFKBP12 has the F36V mutation; amino acids 60-343 …PromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre2_59.F2
Plasmid#60209PurposeExpresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)DepositorInsertCre recombinase (CD207 Human, Synthetic, Bacteriophage P1)
UseTagsFKBP12(F36V) and Nuclear localisation signalExpressionMammalianMutationFKBP12 has the F36V mutation; amino acids 19-59 o…PromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGH50
Plasmid#107254PurposeVipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.DepositorInsertvipp1-gfp-fkbp12
UseSuicide vector for destined for double homologous…TagsGFP and FKBP12ExpressionBacterialMutationPromoternativeAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_FRB-pBirA-HA
Plasmid#83415Purposeproximity-directed biotin labeling on the FRB domain of mTOR in mammalian cellDepositorInsertFRB-pBirA-HA (MTOR Human, Synthetic)
UseTagsHA and pBirAExpressionMammalianMutationPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-dTAG-Puro (BRD4)
Plasmid#91796PurposePITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.DepositorInsertFKBP_F36V-2xHA-P2A-Puro
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-Puro-dTAG (BRD4)
Plasmid#91793PurposePITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertPuro-P2A-2xHA-FKBP_F36V
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-BSD-dTAG (BRD4)
Plasmid#91792PurposePITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertBSD-P2A-2xHA-FKBP_F36V
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromoterlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-dTAG-BSD (BRD4)
Plasmid#91795PurposePITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.DepositorInsertFKBP_F36V-2xHA-P2A-BSD
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 luciferase
Plasmid#26612DepositorInsertfirefly luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TOP luciferase
Plasmid#26611DepositorInserteEF2 5'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
SPELL-TEV Component 1
Plasmid#170489PurposeTEV (N-lobe) fused with iFKBPDepositorInsertTEV(N-lobe), iFKBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pERB111
Plasmid#58281Purposeconstitutive miRempty_Mis12-GFP-FKBP3; inducible mCh-FRBDepositorInsertsempty miRNA
Mis12-GFP-FKBPx3
inducible mCh-FRB
UseTagsGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
SPELL-GDI Component 1
Plasmid#170486PurposeSPELL GDI1 N-lobe fused to iFKBP. Split site is 66.DepositorInsertGDI1(N-lobe)-iFKBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPELL-Lyn Component 1
Plasmid#170484PurposeSPELL Lyn kinase (Constitutively active) N-lobe fused to iFKBP. Split site is 279.DepositorInsertLyn(N-lobe), iFKBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJD427
Plasmid#154371PurposeExpresses FKBP12-XB and SspBcore-FRB from constitutive promotersDepositorInsertproB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPELL-ITSN1(DH-PH) Component 1
Plasmid#170491PurposeSPELL ITSN1(DH-PH) component 1. mCherry-ITSN(N-lobe) fused to iFKBP. Split at loop 4 of DH domain.DepositorInsertmCherry, ITSN(N-lobe), iFKBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
sABE v3.22_C
Plasmid#209554PurposepCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpADepositorInsert2xFKBP-TadA-8e(77-167)-SpCas9(D10A)
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLI-N-sandwich-loxP(K13)
Plasmid#85793PurposeP. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLIDepositorInsertKelch13
UseCre/Lox; P. falciparum genomic integrationTagsdouble 2xFKBP, GFPExpressionMutationPromoternoneAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only