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pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

Plasmid

#236231

Purpose

AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mTagBFP2 fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mCherry-Jph3 WPRE

Plasmid

#236237

Purpose

AAV expression of human Junctophilin3 N-terminally tagged with mCherry

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

Junctophilin 3 (JPH3 Human)

Use

AAV

Tags

mCherry

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mScarlet-CAAX2 WPRE

Plasmid

#236232

Purpose

AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mScarletI

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVenh synapsin-intron-aSYNUCLEIN A53T

Plasmid

#227004

Purpose

AAV transfer plasmid encoding the human A53T mutant α-SYN under the control of the CMVie enhanced synapsin1 promoter

Depositor

Veerle Baekelandt

Article

Van der Perren et al J Vis Exp. 2016 Feb 28;(108):53670. doi:

Insert

alpha synuclein A53T mutant (SNCA Synthetic, Human)

Use

AAV

Mutation

A53T

Promoter

CMVenh synapsin

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-mScarlet-Frame1-Parental-Minicircle

Plasmid

#216125

Purpose

Parental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

sgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD

Use

CRISPR

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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CycK-GFP (1-270AA) in Cilantro (Codon optimized)

Plasmid

#169930

Purpose

Overexpression of 1-270AA CycK-GFP fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Insert

CCNK (CCNK Human)

Use

Lentiviral

Tags

GFP

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2

Plasmid

#110055

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Promoter

Cry1

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-Trono-EFS-Blast-P2A-TurboRFP

Plasmid

#228893

Purpose

Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-control sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179120

Purpose

Expresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

control sgRNA

Use

AAV

Promoter

U6

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)

Plasmid

#221329

Purpose

Dox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

FLAG-IRSp53 and HA-Cdc42(G12V) (Baiap2, Cdc42 Mouse)

Use

Retroviral

Mutation

G12V

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V)

Plasmid

#221330

Purpose

Dox-inducible expression of FLAG-IRSp53-T2A-HA-Rac1 G12V in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

FLAG-IRSp53 and HA-Rac1(G12V) (Baiap2, Rac1 Mouse)

Use

Retroviral

Mutation

G12V

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-P(Per2)-DIO-intron2-Venus-NLS-D2

Plasmid

#110057

Purpose

Per2 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and Venus (Per2 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Promoter

Per2

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pVitro2-hygro-N-myc-hVps34/hVps15-C-V5-his

Plasmid

#24055

Depositor

Jonathan Backer

Article

Yan et al Biochem J. 2009 Feb 1. 417(3):747-55.

Insert

PIK3C3 and PIK3R4 (PIK3C3, PIK3R4 Human)

Use

Bicistronic

Tags

Myc (hVps34) and V5/his (hVps15)

Expression

Mammalian

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-donor(R2Tg)-GFP(intron)

Plasmid

#223248

Purpose

Mammalian expression of the R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(sapI)-CMV-intron-hfCas13d-pA

Plasmid

#195865

Purpose

To express hfCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

high fidelity (hf) version of CasRx (hfCas13d)

Use

AAV

Mutation

Yes: GTGGAATACATTACCAACGTGGTGTACGTG

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mEmerald-STIM1 WPRE

Plasmid

#236234

Purpose

AAV expression of mouse STIM1 internally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mEmerald-Stim1 (Stim1 Mouse)

Use

AAV

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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SVA-lncRNA AK057321 shRNA 1A scramble control

Plasmid

#202835

Purpose

Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1A

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

pLVX-SVA-lncRNA shRNA 1A scramble control

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-Positron2-ST-WPRE

Plasmid

#239080

Purpose

AAV-mediated expression of positive-going voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

Depositor

GENIE Project

Article

Eric R. et al Janelia Figshare 2023

Insert

Positron2-ST

Use

AAV and Cre/Lox

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

R78K N81D D92N W178F

Promoter

hSynapsin1

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203229

Purpose

Cre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al Nat Commun. 2025 Feb 4;16(1):1333. doi:

Insert

Cre-dependent, membrane-localized Optopatch

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-EGFP-Frame0-Parental-Minicircle

Plasmid

#216124

Purpose

Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD

Use

CRISPR

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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