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pCRII-Topo S100a10 in situ probe

Plasmid

#45603

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

s100a10 in situ probe (S100a10 Mouse)

Use

In situ

Mutation

fragment contains bp# 21-275 of BC025044

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pENTR4_FLAG/HA_EWSR1_His6

Plasmid

#26364

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

EWSR1 (EWSR1 Human)

Use

Gateway Cloning

Tags

FLAG/HA and His6

Mutation

delta 657 (stop codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
Vg4-Luc

Plasmid

#16501

Depositor

Bert Vogelstein

Article

Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

Insert

Mad-binding element from Vg

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
3695 pGEX4T-3 MLL AD mut.E

Plasmid

#8867

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Tags

GST

Expression

Bacterial

Mutation

ILPSDIMDFVL --> ILPADIMDFVL

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
PEmaxNG-_2star_IVT

Plasmid

#227678

Purpose

plasmid for SpCas9-NG-PEmax** mRNA IVT

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

NG-PEmax_2_star

Use

CRISPR

Tags

no

Expression

Mammalian

Promoter

T7

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-FEN1

Plasmid

#215129

Purpose

Expresses mCherry-FEN1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

FEN1 (FEN1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-LIG1

Plasmid

#215131

Purpose

Expresses mCherry-LIG1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

LIG1 (LIG1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_071

Plasmid

#164558

Purpose

Higher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

Insert

sgRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pH-Ubi-miniSdd7-nCas9-PBE

Plasmid

#204856

Purpose

For cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd7-nSpCas9(D10A)-UGI

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Promoter

ZmUbi-1, OsU3, 35S

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Linear Tau 0N4R 3X Flag tag

Plasmid

#194171

Purpose

Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-Associated Protein Tau 0N4R

Tags

3X Flag

Expression

Mammalian

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMax_GFP_C289T

Plasmid

#177826

Purpose

Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

GFP_C289T

Expression

Mammalian

Mutation

C289T

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMax_mPlum_C207G/T208C

Plasmid

#177831

Purpose

Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mPlum_C207G/T208C

Expression

Mammalian

Mutation

C207G + T208C

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-SARS-CoV-2 5'UTR-Luciferase

Plasmid

#191480

Purpose

Luciferase reporter for SARS-CoV-2 5' UTR

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

SARS-CoV-2 5' UTR

Use

Lentiviral and Luciferase

Mutation

contains SARS-CoV-2 5' UTR

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HT028_FCMV_CheRiff_EGFP

Plasmid

#178821

Purpose

Expresses a blue-light activated cationic channelrhodopsin CheRiff carrying an EGFP tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

CheRIff_EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS/D614G

Plasmid

#164566

Purpose

SARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)

Depositor

Priyamvada Acharya

Article

Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

Insert

Spike (S-GSAS-D614G variant)

Tags

2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Orf9b L52D

Plasmid

#165129

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Orf9b L52D

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp14-mCherry

Plasmid

#165137

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp14 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp14-EGFP

Plasmid

#165119

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp14 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Nsp7

Plasmid

#165112

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp7 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2-TetR-neoR

Plasmid

#153310

Purpose

Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

Depositor

Spyros Petrakis

Article

Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

Insert

TetR

Use

Transposon

Promoter

SV40

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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