We narrowed to 11,803 results for: nsf

1. AAV-mCREB

   Plasmid

   #68551

   Purpose

   AAV expression of mCREB

   Depositor

   Eric Nestler

   Article

   Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

   Insert

   mCREB
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S133A

   Promoter

   CMV

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

2. AAV2/9-CW3SL-mCherry-DrTrkB

   Plasmid

   #184002

   Purpose

   AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

   Depositor

   Dmitry Gerashchenko, Vladislav Verkhusha

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrTrkB (NTRK2 Human)
   

   Use

   AAV

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

   Plasmid

   #129530

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIC3Nme
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-pMecp2-dSaCas9-VP160-pU6-sgRNA

   Plasmid

   #158987

   Purpose

   Vector D encodes pAAV-pMecp2-dSaCas9-VP160-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-VP160
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   pMecp2

   Available Since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CMV.RGS4.C-HA

   Plasmid

   #154847

   Purpose

   Expresses murine RGS4 protein with HA-tag at C-terminus

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Regulator of G protein Signaling 4 (Rgs4 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CAG-flex-lck-smV5

   Plasmid

   #196423

   Purpose

   Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter; no cell-type specificity

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   Lck-smV5
   

   Use

   AAV and Cre/Lox

   Tags

   Lck

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS1988

   Plasmid

   #49119

   Purpose

   AAV plasmid with CAGGS promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-CAGGS-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV-CMV-NLS-AcrIIA4

   Plasmid

   #113038

   Purpose

   AAV vector for expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SV40 NLS-AcrIIA4
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3

   Plasmid

   #121976

   Purpose

   transfection studies in eukaryotic cells such as COS-7 cells

   Depositor

   Christophe Erneux

   Article

   De Smedt et al J Biol Chem. 1996 Apr 26;271(17):10419-2

   Insert

   Type I Inositol-1,4,5-trisphoosphate 5-phosphatase (INPP5A Human)
   

   Expression

   Mammalian

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiMutB3GALT5catdead-blast

    Plasmid

    #220869

    Purpose

    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    encodes D156A and D243A mutations; also, nucleoti…

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FDIO-COX4-dAPEX2

    Plasmid

    #117178

    Purpose

    Plasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    COX4-dAPEX2
    

    Use

    AAV; Flp/frt

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cerulean-GalT

    Plasmid

    #11930

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Cole et al Science. 1996 Aug 9. 273(5276):797-801.

    Insert

    GalT (GALT Human)
    

    Expression

    Mammalian

    Mutation

    Amino acids 1-60 of the human galactosyltransfera…

    Available Since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-C-Solo-mStayGold-PXN

    Plasmid

    #227316

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    PXN Homology Arms flanking a mStayGold Tag (PXN Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV_Actb HMEJ donor

    Plasmid

    #97318

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZac2.1 U6-SaDMDR7-U6-SaDMDL2

    Plasmid

    #78603

    Purpose

    AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SaDMDR7-U6-SaDMDL2
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pscAAV-Tyr

    Plasmid

    #135616

    Purpose

    Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRosa26-TG

    Plasmid

    #40026

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (ATG)
    beta-globin intron
    Neo
    GFP
    diphteria toxin A
    

    Expression

    Mammalian

    Mutation

    deleted nucleotides 1-274 and insertion of FRT, …

    Promoter

    CAG (chicken beta globin promoter and CMV enhance…

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CaMKII-soCoChR-GFP

    Plasmid

    #107711

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EFS-DIO-GRAB_OTmut

    Plasmid

    #185389

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-4mtD3cpv

    Plasmid

    #181874

    Purpose

    Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    4mtD3cpv
    

    Use

    AAV and Adenoviral

    Expression

    Mammalian

    Mutation

    the 4mtD3cpv CDS differs from the recorded GeneBa…

    Promoter

    CaMKIIa

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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