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pAc5.1B-lambdaN-HA-DmLSm5_A

Plasmid

#145890

Purpose

Insect Expression of DmLsm5

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLsm5 (CG6610 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm7_A

Plasmid

#145891

Purpose

Insect Expression of DmLSm7

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm7 (LSm7 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-zfKitlga

Plasmid

#168199

Purpose

Express zebrafish Kitlga, mRNA synthesis

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlga (kitlga Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Mutation

F198L - please see depositor comments

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-zfTpo

Plasmid

#168198

Purpose

Express zebrafish thrombopoietin (Tpo), mRNA synthesis

Depositor

Petr Bartunek

Article

Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

Insert

Thrombopoietin (thpo Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm3_A

Plasmid

#145888

Purpose

Insect Expression of DmLSm3

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm3 (LSm3 Fly)

Expression

Insect

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hmga2 shMut wt

Plasmid

#52036

Purpose

encodes shMut HMGA2

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

shRNA binding site mutated

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hmga2 shMut m7

Plasmid

#52037

Purpose

encodes shMut HMGA2 m7

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

shRNA binding site mutated, all seven let-7 sites…

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hmga2 shMut ATG wt

Plasmid

#52038

Purpose

encodes shMut HMGA2 ATG wt

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

shRNA binding site mutated, first ATG mutated

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hmga2 shMut ATG m7

Plasmid

#52039

Purpose

encodes shMut HMGA2 ATG m7

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

shRNA binding site mutated, first ATG mutated, al…

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pαH-S-GSAS-OMICRON.BA.2-RBD

Plasmid

#184830

Purpose

Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

Depositor

Priyamvada Acharya

Article

Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

Insert

S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)

Tags

6X His tag

Expression

Mammalian

Mutation

SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pB-Halo,IRES-eGFP

Plasmid

#164519

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5_miniTurbo-C12orf49

Plasmid

#155111

Purpose

transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

C12orf49 (SPRING1 Human)

Tags

miniTurbo

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLENTI EF1alpha FLAG/HA-PALI1

Plasmid

#114445

Purpose

Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human PALI1 coding sequence

Depositor

Adrian Bracken

Article

Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

Insert

PALI1 (LCOR Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSLED.GluA2

Plasmid

#193017

Purpose

GluA2 (Gria2) flip/flop reporter (hSyn promoter, Gria2 flip/flop exons, bichromatic reporter)

Depositor

Jonathan Ling

Article

Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

Insert

Bichromatic reporter (EGFP and dsRed-Express2)

Use

AAV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shWRN2

Plasmid

#125782

Purpose

constitutive expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 Luciferase

Plasmid

#117072

Purpose

single guide RNA targeting Luciferase

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

Luciferase

Use

CRISPR

Promoter

hU6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 PSMB5 guide 1

Plasmid

#117073

Purpose

single guide RNA targeting PSMB5; guide 1

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

LMPX (PSMB5 Human)

Use

CRISPR

Promoter

hU6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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47 pLIB_GST

Plasmid

#160272

Purpose

N-term 3C cleavable GST tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

GST

Expression

Insect

Promoter

polyhydrin

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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52 pLIB_3C_C_STREP

Plasmid

#160300

Purpose

C-term 3C cleavable 2xStrepII tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

2xStrepII

Expression

Insect

Promoter

polyhydrin

Available Since

Nov. 15, 2020

Availability

Academic Institutions and Nonprofits only

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40 pCOLI_K_MBP

Plasmid

#160183

Purpose

N-term MBP 3C cleavable Kan resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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