We narrowed to 13,072 results for: BASE;
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Plasmid#201956PurposeMammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNADepositorInsertbpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianPromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE
Plasmid#118274PurposeExpresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoterDepositorInsertsbPAC(WT)
SthK
UseAAVTagsHis tag, P2A, mCherry, and myc tagExpressionMammalianPromoterCamK IIAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-BRCT2-Linker-eGFP
Plasmid#176085PurposeEGFP fused to the C-terminus of a BRCT2 domain & a hygromycin resistance cassetteDepositorAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-BRCT1-Linker-eGFP
Plasmid#176074PurposeEGFP fused to the C-terminus of a BRCT1 domain & a hygromycin resistance cassetteDepositorAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSbi-pur-EGFP-KSR1-CA3 (N-KSR)
Plasmid#217769PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseSleeping beauty transposase-compatible genome ins…TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400PromoterEF-1alpha promoterAvailable SinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A
Plasmid#212949PurposeExpresses TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A in mammalian cellsDepositorInsertTALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A (mt-Rnr1 Synthetic)
ExpressionMammalianAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRGEN-CMV-VPR-L1-CjCas9 V-D
Plasmid#169913PurposeExpression of a VPR-dCjCas9 fusion protein to activate gene expression.DepositorInsertdCjCas9
UseCRISPRTags2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)ExpressionMammalianMutationD8A, H559A in cjCas9PromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-CACNA1E KI
Plasmid#131481PurposeEndogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)DepositorAvailable SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRE3GV-SpdCas9-Dnmt3A/3L-WT
Plasmid#222502PurposeExpresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherryDepositorInsertsS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
mCherry
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterTRE3GV and hPGKAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PPO-Venus
Plasmid#139502PurposeExpresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.DepositorInsertparapinopsin
TagsVenus tag following 3x Ala linkerExpressionMammalianAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)
Plasmid#217767PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseProtein purification (6xhis tag, tev site, sumo3 …TagssfGFPExpressionBacterialMutationCA3 domain aa 317-400PromoterT7 promoterAvailable SinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-CMV-EGFP-PolB-PAMmut-Hygro
Plasmid#176086PurposeEGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassetteDepositorInsertPolB (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutation in the PAM site used by PolBKOg1PromoterCMVAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneoLuc-TEAD4
Plasmid#128335PurposeVector for LUMIER assayDepositorAvailable SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107576PurposeB. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic BiologyTagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterpdh promoter Bacillus megaterium DSM319Available SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA
Plasmid#97308PurposeHMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.DepositorInsertActb HMEJ donor
UseAAV and Mouse TargetingExpressionMammalianPromoterU6, EF1aAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP HAT
Plasmid#179547Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mScarlet-gamma9
Plasmid#140097PurposeExpresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocationDepositorInsertGNGT2
TagsN-terminal mScarlet tagExpressionMammalianPromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107582PurposeB. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic Biology; E. coli and bacillus shuttle v…TagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterfba promoter Bacillus megaterium DSM319Available SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA
Plasmid#97311PurposeMMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.DepositorInsertActb MMEJ donor
UseAAV and Mouse TargetingExpressionMammalianPromoterU6, EF1aAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PA-mCit-BAD
Plasmid#113459PurposeProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)DepositorInsertBCL2 associated agonist of cell death (BAD Human)
TagsProteinA-mCitrineExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only