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Showing: 8481 - 8500 of 17687 results
  1. pCOTS-pyl-GFP(35TAG)

    Plasmid
    #92047
    Purpose
    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.
    Depositor
    Inserts
    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Replicative expression plasmid for cyanobacteria …
    Tags
    Histag
    Mutation
    Tyrosine in position 35 of the GFP was mutated f…
    Promoter
    LeuP (native S. elongatus promoter), PpsbII, and …
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pENTR-DMD-Donor

    Plasmid
    #60605
    Purpose
    Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.
    Depositor
    Insert
    Hygromycin resistant gene
    Use
    Knock-in donor vector
    Expression
    Mammalian
    Available Since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pCDF-Az

    Plasmid
    #197101
    Purpose
    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine
    Depositor
    Insert
    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    Expression
    Bacterial
    Mutation
    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…
    Available Since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pET24a-VHH-2xTS

    Plasmid
    #109419
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Insert
    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 8481 - 8500 of 17687 results