174,421 results
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Plasmid#103864PurposedPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.DepositorInsertsUseCRISPRTagsGSGGGGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…Available SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only
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pSNAPf-H2B Control Plasmid
Plasmid#101124PurposeExpression of SNAP-tagged Histone H2B in mammalian cellsDepositorInserthistone cluster 1 H2B family member j (H2BC11 Human)
TagsSNAP-tag (SNAPf)ExpressionMammalianPromoterCMVAvailable SinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterA. nidulans gpdA promoter with Hammerhead ribozym…Available SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-EGFP-C1-Flag-mFmr1(wt)
Plasmid#87929PurposeExpresses mouse FMRP-EGFP fusion, which can be phosphorylated on ser-499.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS
Plasmid#86677PurposeExpresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.DepositorInsertEGFP-NLS
UseLentiviralAvailable SinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-EGFP BlastR
Plasmid#84383PurposeLentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsEGFPPromoterCMV immediate earlyAvailable SinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-NuMA
Plasmid#81029PurposeExpresses human NuMA with N-terminal EGFP tag in mammalian cellsDepositorInsertHomo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-IDH2-R172K-FLAG
Plasmid#66807PurposeTet-inducible expression of mutant IDH2 in mammalian cellsDepositorInsertIDH2 (IDH2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationR172KPromoterCMVAvailable SinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
moxCerulean3
Plasmid#68066PurposeC-terminal fusion to moxCerulean3DepositorTypeEmpty backboneTagsmoxCerulean3ExpressionMammalianPromoterCMVAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-dCas9-VPR
Plasmid#63801PurposepAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vectorDepositorInsertdCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionYeastPromoterGPDAvailable SinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only