We narrowed to 18,864 results for: IRE;

1. pMALcPP-MS2cp

   Plasmid

   #203351

   Purpose

   Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

   Depositor

   Thomas Cech

   Article

   Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

   Insert

   MS2 coat protein
   

   Use

   Tags

   Hx6-MBP

   Expression

   Bacterial

   Mutation

   V75E and A81G, to prevent capsid assembly

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCPP5238

   Plasmid

   #128711

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopG1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs_NMNAT3-FLAG

   Plasmid

   #133261

   Purpose

   Express FLAG-tagged NMNAT3 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   NMNAT3 (NMNAT3 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-KLF7-200-GFPfr1

   Plasmid

   #169799

   Purpose

   THe donor vector for the KLF7 gene locus.

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   Donor sequence to KLF7 neighbouring region for HR
   

   Use

   Hr donor vector for human actb gene.

   Tags

   Expression

   Mutation

   Partial sequence of GFP is inserted the sequence …

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPRv2-KLF7-1

   Plasmid

   #169797

   Purpose

   Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   guide RNA for KLF7 neighbouring region
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21-pelB-LaG-16-3xFLAG-LaG-2

   Plasmid

   #172765

   Purpose

   Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-16-3xFLAG-LaG-2
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-IP-EGFP-ULK1(K46N)

   Plasmid

   #38197

   Depositor

   Noboru Mizushima

   Article

   Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

   Insert

   a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed Lysine 46 to Asparagine

   Promoter

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

8. shLacZ

   Plasmid

   #42559

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   shLacZ
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pLKO mouse shRNA 2 raptor

   Plasmid

   #21340

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

   Insert

   raptor shRNA 2 (Rptor Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pMAL

    Plasmid

    #161783

    Purpose

    Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and GFP.

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Bidirectional minhCMV and hPGK

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. XLone-SOX18

    Plasmid

    #163822

    Purpose

    expresses human SOX18 upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    SOX18 (SOX18 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CCR5_gRNA_pX459

    Plasmid

    #192268

    Purpose

    gRNA expression for CRISPR Genome Editing of CCR5

    Depositor

    Shantanu Chowdhury

    Article

    Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

    Insert

    CCR5 guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR1A hu_ncOGT WT

    Plasmid

    #154281

    Purpose

    Entry vector encoding non-tagged O-GlcNAc Transferase

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    WT OGT

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. FUW-TetO-Dmrt1

    Plasmid

    #41083

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

    Insert

    Dmrt1 (Dmrt1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO + minimal CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCPP5951

    Plasmid

    #128719

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

    Insert

    hopR1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG-6

    Plasmid

    #172741

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-6
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDUP1

    Plasmid

    #223516

    Purpose

    inactive cogfp under control of Ptet, cogfp under control of Ptac

    Depositor

    Yolanda Schaerli

    Article

    Ljiljana et al eLife13:RP97216

    Inserts

    CoGFP
    CoGFP inactivated
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    anti-dimerization mutations S129G, 22 C154S, D156…

    Promoter

    Ptac, Ptac and Ptet, and Ptet

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. NylB'-SCY_pET21b

    Plasmid

    #215417

    Purpose

    Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB'-SCY
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R187S/F264C/D370Y

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRcCMV Cep164 (Nigg CW325)

    Plasmid

    #41148

    Depositor

    Erich Nigg

    Article

    Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

    Insert

    Cep164 (CEP164 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

20. EF1a_SPIB_P2A_Hygro_Barcode

    Plasmid

    #120483

    Purpose

    Barcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SPIB (SPIB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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