We narrowed to 11,803 results for: nsf

1. pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

   Plasmid

   #125236

   Purpose

   Expresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
   

   Use

   AAV

   Tags

   Flag, V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Syn-ChromeQ-GFP

   Plasmid

   #123317

   Purpose

   AAV-mediated expression of ChromeQ-GFP under the Syn promoter.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeQ-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

   Promoter

   Syn

   Available Since

   March 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSKA397

   Plasmid

   #80380

   Purpose

   mini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.

   Depositor

   Mustafa Khammash

   Article

   Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

   Inserts

   cat
   metE
   

   Use

   Synthetic Biology; Mini-tn7 delivery vector

   Expression

   Bacterial

   Promoter

   cat promoter and cpcG2Δ59

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CAG-DIO-erHRP(N175S mutant)

   Plasmid

   #79906

   Purpose

   Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

   Depositor

   Joshua Sanes

   Article

   Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

   Insert

   erHRP(N175S mutant)
   

   Use

   AAV and Cre/Lox

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   N175S mutation

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. CSAC-Bmal1

   Plasmid

   #168296

   Purpose

   Expresses sgRNA in mammalian cells

   Depositor

   Han Kyoung Choe

   Article

   Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

   Insert

   hU6-sgBmal1-1-hU6-sgBmal1-3
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   U6, hSyn

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP7-bio

   Plasmid

   #47735

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP7 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP7
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-eYFP-3x-miR708-5p-TS

   Plasmid

   #117381

   Purpose

   An AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   EYFP + 3 copies of miR708: CCCAGCTAGATTGTAAGCTCCTT
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p164-pAAV-mDlx-FLEX-mScarlet

   Plasmid

   #204689

   Purpose

   Fluorescent reporter under the control of the Dlx promotor

   Depositor

   Lynette Lim

   Article

   Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

   Insert

   mScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   mScarlet palmitoylation

   Expression

   Mammalian

   Promoter

   mDlx

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC8_GWs-Lox (VE5589)

   Plasmid

   #163769

   Purpose

   Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   no insert
   

   Tags

   no tag

   Expression

   Insect

   Promoter

   PH

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP789_αGCN4-DNMT3A-CO

    Plasmid

    #211783

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-DNMT3A
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAav-MCS-PQS2-3xHA

    Plasmid

    #84917

    Purpose

    rAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

    Insert

    LOX-PGK-NEO-LOX
    

    Use

    AAV

    Tags

    PQS2 3xHA

    Promoter

    mPGK

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

    Plasmid

    #194688

    Purpose

    hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_04-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Promoter

    hSyn1

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSP5-bio

    Plasmid

    #47718

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP5 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP5
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-RO1.7-Lrat

    Plasmid

    #206345

    Purpose

    AAV plasmid expressing LRAT in photoreceptors

    Depositor

    Connie Cepko

    Article

    Xue et al Proc Natl Acad Sci U S A. 2023 Jun 6;120

    Insert

    Lrat (Lrat Mouse)
    

    Use

    AAV

    Promoter

    1.7kb human red opsin

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBL_DK202

    Plasmid

    #156426

    Purpose

    Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (aa 42-275)
    

    Tags

    EGFP-His

    Expression

    Mammalian

    Mutation

    aa 42-275

    Promoter

    CMV

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA

    Plasmid

    #37089

    Purpose

    Expresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    ChETA-TdTomato
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    TdTomato

    Promoter

    EF1a

    Available Since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKII-nirButterfly

    Plasmid

    #136591

    Purpose

    AAV plasmid for CaMKII-driven expression of a genetically-encoded voltage indicator nirButterfly

    Depositor

    Vladislav Verkhusha

    Article

    Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

    Insert

    nirButterfly
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199368

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-SUMOstar-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. RhopH3-bio

    Plasmid

    #47781

    Purpose

    Expresses enzymatically monobiotinylated full-length RhopH3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RhopH3
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Susd4(3.7)-EGFP-sv40

    Plasmid

    #184413

    Purpose

    AAV expression of tTA from mouse sushi domain containing 4 (Susd4) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Susd4 gene promoter-EGFP (Susd4 Mouse)
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Mouse Susd4 gene

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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