We narrowed to 10,356 results for: plasmids 101

1. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

   Plasmid

   #125712

   Purpose

   Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   eOPN3
   

   Use

   AAV

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 331-429

   Promoter

   short version of αCamKinase II promoter (0.4kb)

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60228

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV phSyn1(S)-DreO-bGHpA

   Plasmid

   #50363

   Purpose

   Can be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Has Service

   AAV Retrograde and AAV5

   Insert

   DreO recombinase
   

   Use

   AAV

   Tags

   none

   Expression

   Mutation

   Sequence optimized for expression in mammalian ce…

   Promoter

   hSyn1

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

   Plasmid

   #113163

   Purpose

   A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60227

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZ M2rtTA_CAGG TetON-Sox10 with GFP

   Plasmid

   #115241

   Purpose

   RMCE donor vector for inducible expression of SOX10 and GFP and constitutive expression of m2rtTA (generated from plasmid #112668)

   Depositor

   Catherine Verfaillie

   Article

   Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

   Inserts

   SOX10 (SOX10 Human)
   eGFP
   

   Use

   Tags

   GFP (separate polypeptide)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pscALPSpuro-RsACE2

   Plasmid

   #159665

   Purpose

   Expresses Rhinolophus sinicus ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   rhinolophus sinicus ACE2 (ACE2 rhinolophus sinicus)
   

   Use

   Lentiviral

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV phSyn1(S)-FlpO-bGHpA

   Plasmid

   #51669

   Purpose

   Can be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter.

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Has Service

   AAV Retrograde

   Insert

   FlpO recombinase gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-GRAB_DA1m

   Plasmid

   #113049

   Purpose

   express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

   Has Service

   AAV9

   Insert

   GPCR activation based DA sensor GRAB_DA1m (DRD2 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Tnnt2-GFP-LA

    Plasmid

    #206198

    Purpose

    Expresses GFP-tagged Lamin-A specifically in cardiomyocytes by the Tnnt2 promoter

    Depositor

    Yuxuan Guo

    Article

    Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

    Insert

    Lmna (LMNA Human)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Tnnt2

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tet-O-FUW-EGR3-P2A-mCherry

    Plasmid

    #203861

    Purpose

    Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGR3 (EGR3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-SOUL-P2A-tdTomato

    Plasmid

    #153019

    Purpose

    Step-function opsin with ultra-high light sensitivity (SOUL). Capable to be used for noninvasive stimulation in mice and minimally invasive stimulation in macaque.

    Depositor

    Guoping Feng

    Article

    Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

    Insert

    SOUL
    

    Use

    AAV

    Tags

    P2A-tdTomato

    Expression

    Mammalian

    Mutation

    C128S/D156A/T159C

    Promoter

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

    Plasmid

    #125713

    Purpose

    Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

    Has Service

    AAV1 and AAV5

    Insert

    eOPN3
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 331-429

    Promoter

    hSyn1

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBFC0996

    Plasmid

    #186695

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    crRNA
    Tn7 transposon
    2xLguI Cargo Stuffer
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-hSOX2-17

    Plasmid

    #193350

    Purpose

    Retroviral vector expressing human SOX2-17 chimeric transcription factor (super-SOX)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    SOX2-17 (SOX2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    SOX2-17 is engineered highly cooperative chimeric…

    Promoter

    5'LTR

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOTTC763 - pX458 with rat Rosa26 gRNA A

    Plasmid

    #113161

    Purpose

    A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat Rosa26
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-DIO-PPO-Venus

    Plasmid

    #139505

    Purpose

    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Has Service

    AAV8 and AAV9

    Insert

    parapinopsin
    

    Use

    AAV

    Tags

    Venus tag following 3x Ala linker

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIGF1_P1 Promoter-FLuc

    Plasmid

    #154266

    Purpose

    Firefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    IGF-1 canonical P1 Promoter (IGF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human IGF-1 P1 Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tet-O-FUW-FOSL2-P2A-mCherry

    Plasmid

    #203860

    Purpose

    Tet-inducible lentiviral plasmid expressing FOSL2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    FOSL2 (FOSL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre

    Plasmid

    #112683

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-iCre and Nuclear localization signal

    Expression

    Mutation

    Promoter

    CMV-IE

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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