We narrowed to 12,359 results for: NSI

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pAAV-Ef1a-Coff/Fon-GCaMP6M

Plasmid

#137121

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-K298M-Y419F-Y530F

Plasmid

#140321

Purpose

Expresses Flag-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

K298M-Y419F-Y530F

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-BFP

Plasmid

#137129

Purpose

Intersectional viral expression of BFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-NMHC-IIA-S1916A

Plasmid

#101040

Purpose

Expresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.

Depositor

Tom Egelhoff

Article

Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311

Insert

Non-muscle myosin IIA heavy chain (MYH9 Human)

Tags

GFP

Expression

Mammalian

Mutation

S1916A, S1917A

Promoter

CMV

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 S_nostop

Plasmid

#152988

Purpose

Gateway-compatible Entry vector, with insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-BRD9-PGK-Puro-IRES-GFP

Plasmid

#75114

Purpose

Retroviral expression plasmid encoding wild type human BRD9

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
FUG-T2A-Pten

Plasmid

#140738

Purpose

Lentiviral vector to overexpress human PTEN and GFP

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-PTA-PTEN (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING-354STOP

Plasmid

#102589

Purpose

Lentivector to express STING delta 354 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta 354

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-BFP

Plasmid

#137131

Purpose

Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-oScarlet

Plasmid

#137137

Purpose

Intersectional viral expression of oScarlet in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E95D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGLS3-5xHRE-p53(K120R)

Plasmid

#72554

Purpose

Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

p53 (TP53 Human)

Tags

5XHRE

Expression

Mammalian

Mutation

K120R

Promoter

E1B minimal promoter

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF846

Plasmid

#88355

Purpose

Donor Vector containing ZNF846 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF846 (ZNF846 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-sRGECO

Plasmid

#137127

Purpose

Intersectional viral expression of sRGECO in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-p53DD

Plasmid

#196582

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

PE2-P2A-mp53DD

Expression

Mammalian

Mutation

Δ40-903

Promoter

CMV

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2V678F-VSV

Plasmid

#139349

Purpose

Expression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)

Depositor

Sandra Pellegrini

Article

Gakovic et al J Biol Chem. 2008 Jul 4;283(27):18522-9.

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-V678F

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF655

Plasmid

#88578

Purpose

Donor Vector containing ZNF655 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF655 (ZNF655 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-T1977R

Plasmid

#69011

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-T1977R (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Threonine 1977 to Arginine

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only
pCAGGS-SARS2-S-D614 (NC-flag)

Plasmid

#156418

Purpose

Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING-V155M S366A

Plasmid

#102593

Purpose

Lentivector to express STING V155M S366A and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M S366A

Promoter

SFFV

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF736

Plasmid

#101602

Purpose

Donor Vector containing ZNF736 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF736 (ZNF736 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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