We narrowed to 11,712 results for: NSI

1. pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP

   Plasmid

   #137141

   Purpose

   Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-ChR2(ET/TC)-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   E123T, T159C

   Promoter

   nEF

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EMTB-TurboID-V5-puro

   Plasmid

   #190741

   Purpose

   A lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   EMTB-TurboID-V5 (RPS14 EMTB is human ensconsin; TurboID is engineered BirA from E.coli, Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Microtubule binding-domain of ensconsin (amino ac…

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS

   Plasmid

   #145269

   Purpose

   Mammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   TBP(38Q)-IDR (TBP Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Gal4-HA-HIF1α(530-652)

   Plasmid

   #24887

   Depositor

   William Kaelin

   Article

   Ivan et al Science. 2001 Apr 20. 292(5516):464-8.

   Insert

   hypoxia inducible factor1alpha (HIF1A Human)
   

   Tags

   Gal4dbd and HA-tag

   Expression

   Mammalian

   Mutation

   partial HIF1alpha cDNA (encoding aa 530-652)fused…

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN

   Plasmid

   #99049

   Purpose

   Transcription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain

   Depositor

   Barak Cohen

   Article

   Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

   Inserts

   ACT1 promoter (ACT1 Budding Yeast)
   mCherry
   Zif268 DNA binding Domain
   Estrogen response domain (ESR1 Human)
   

   Expression

   Yeast

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-Con/Foff 2.0-GCaMP6M

   Plasmid

   #137120

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO_HA.NWS.VSVg_NGFR

   Plasmid

   #158243

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HA.NWS.VSVg

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nEF-Coff/Fon-NpHR3.3-EYFP

   Plasmid

   #137154

   Purpose

   Intersectional viral expression of NpHR3.3-EYFP in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-NpHR3.3-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   W179F

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Archon2-KGC-GFP-ER2-WPRE

   Plasmid

   #108424

   Purpose

   Archon2 fluorescent voltage reporter in mammalian expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon2-KGC-EGFP-ER2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

    Plasmid

    #210710

    Purpose

    This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    M11

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP

    Plasmid

    #137148

    Purpose

    Intersectional viral expression of Arch3.3-EYFP in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-Arch3.3-p2a-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    n/a

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIP-SFFV-mtagBFP-2A STING V155M 368STOP

    Plasmid

    #102596

    Purpose

    Lentivector to express STING V155M delta 368 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M delta 368

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-nEF-Con/Fon-ChR2-mCherry

    Plasmid

    #137142

    Purpose

    Intersectional viral expression of ChR2-mCherry in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-ChR2-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    H134R

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO_HA.FLAG.VSVg_NGFR

    Plasmid

    #158245

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.FLAG.VSVg

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX304_zeo_mmMavs_V5

    Plasmid

    #160100

    Purpose

    Expresses murine Mavs in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Mavs (Mavs Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-nEF-Con/Foff 2.0-ChR2-EYFP

    Plasmid

    #137163

    Purpose

    Intersectional viral expression of ChR2-EYFP in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-ChR2-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    H134R

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOmnibac_ZZ_linker_ybbR_Tau

    Plasmid

    #159717

    Purpose

    Expresses tau in Sf9 cells with a ZZ affinity tag and a ybbR tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    Microtubule-associated protein tau (MAPT Human)
    

    Tags

    ZZ affinity tag, ybbR tag

    Expression

    Insect

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-nEF-Coff/Fon-ChR2-mCherry

    Plasmid

    #137144

    Purpose

    Intersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-ChR2-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    H134R

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO_NWS.FLAG.VSVg_NGFR

    Plasmid

    #158233

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    NWS.FLAG.VSVg

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223 SARS-CoV-2 ORF3B_nostop

    Plasmid

    #149320

    Purpose

    Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF3B
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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