We narrowed to 26,138 results for: Vit

1. pLentiPGK Puro DEST JNKKTR AE Clover

   Plasmid

   #90240

   Purpose

   Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (AE mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ265G2

   Plasmid

   #164723

   Purpose

   Gateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   A3A(Y130F)-ziSpyMac(D10A)-UGI)
   

   Use

   CRISPR; Gateway compatible a3a/y130f-ispymaccas9-…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. UbG76V-eGFP-V5His_15aa

   Plasmid

   #23969

   Depositor

   Nico Dantuma

   Article

   Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

   Insert

   Ub-G76V-eGFP-V5HIS_15aa (UBB Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   G76V mutation in Ubiquitin. Ub fused to N-termin…

   Promoter

   Available since

   April 16, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-mCherry-TSC2(AA)-NLS

   Plasmid

   #192443

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNAIIIB rac1 QL

   Plasmid

   #61633

   Purpose

   mammalian expression of rac1 constitutively active mutant

   Depositor

   JS Gutkind

   Article

   Coso et al Cell. 1995 Jun 30;81(7):1137-46.

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q61L, constitutively active

   Promoter

   CMV

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

6. AAV-SYN_mMaroon-KIX-mMaroon

   Plasmid

   #137009

   Purpose

   AAV backbone, mMaroon-KIX acceptor for R-CREB

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

   Insert

   KIX domain (Crebbp Rat)
   

   Use

   AAV

   Tags

   mMaroon

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. NEK10 gRNA (BRDN0001162335)

   Plasmid

   #75636

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NEK10

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NEK10 (NEK10 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. LightR-bRaf-mVenus

   Plasmid

   #162154

   Purpose

   Expresses LightR-bRaf-mVenus in mammalian cells

   Depositor

   Andrei Karginov

   Article

   Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

   Insert

   LightR-bRaf-mVenus
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Valine 600 is substituted with Glutamic acid

   Promoter

   CMV

   Available since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

   Plasmid

   #163698

   Purpose

   AAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortex

   Depositor

   Gina Turrigiano

   Article

   Trojanowski et al Neuron. 2021 Feb 17;109(4):663-676.e5. d

   Insert

   CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII fragment

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. VRQR-HF1 variant

    Plasmid

    #138563

    Purpose

    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSG5-HA-ERR3 S57,81,219A

    Plasmid

    #37850

    Depositor

    Rebecca Riggins

    Article

    Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

    Insert

    Estrogen-related receptor gamma (Esrrg Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Serines 57, 81, and 219 to Alanine (AGC t…

    Promoter

    SV40

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pDEST_FLAG-HUWE1_BH3-4E

    Plasmid

    #187144

    Purpose

    mammalian cell expression of FLAG-HUWE1 V1976E/V1780E/L1983E/M1987E

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    V1976E/V1780E/L1983E/M1987E

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST_FLAG-HUWE1_delta3291-3298

    Plasmid

    #187125

    Purpose

    mammalian cell expression of FLAG-HUWE1 delta3291-3298

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    delta3291-3298

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PHKA1 gRNA (BRDN0001144977)

    Plasmid

    #77838

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PHKA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PHKA1 (PHKA1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

    Plasmid

    #181814

    Purpose

    Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    Muc5b D1 (Muc5b Mouse)
    

    Use

    Tags

    TEV cleavage site followed by 6xHistidine tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKT2/CAGXSP/PalmNanoLuc

    Plasmid

    #182969

    Purpose

    Stably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.

    Depositor

    Masamitsu Kanada

    Article

    Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

    Insert

    PalmNanoLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. MDH1-shSHP2-3-H2Kb2

    Plasmid

    #17854

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    shSHP2 (PTPN11 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTER E2F2shRNA human

    Plasmid

    #66884

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F2

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F transcription factor 2 (E2F2 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 Tet

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

    Plasmid

    #124660

    Purpose

    reporter protein which emits bright blue fluorescence

    Depositor

    Yutaka Kuroda

    Article

    Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

    Insert

    Gaussia luciferase-C9D-TGinsert
    

    Use

    Tags

    IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

    Expression

    Bacterial

    Mutation

    changed glutamic acid 100 to alanine, and glycine…

    Promoter

    T7 promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330 N-FKBP-Cas9

    Plasmid

    #162727

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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