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EF1a_ASCL1_P2A_Hygro_Barcode

Plasmid

#120427

Purpose

Barcoded lentiviral vector to express ASCL1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-oct4

Plasmid

#20323

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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TFORF2281

Plasmid

#142929

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MYBBP1A (MYBBP1A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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tCRMod-GGSGGS9

Plasmid

#111762

Purpose

Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-GGSGGS9-mRuby2 fusion

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a Halotag-CNA35

Plasmid

#131128

Purpose

Halotag-CNA35 peptide for Collagen labelling

Depositor

Samantha Stehbens

Article

Ju et al bioRxiv 2022

Insert

Halotag-CNA35

Tags

Halotag

Expression

Bacterial

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFV2/myc-His

Plasmid

#127491

Purpose

Expresses myc-tagged NDUFV2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFV2 (NDUFV2 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 2

Plasmid

#128571

Purpose

for cloning identified individual SPECS

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagRFP

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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∆DUSP6

Plasmid

#27977

Depositor

Igor Astsaturov

Article

Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

Insert

DUSP6 (DUSP6 Human)

Use

Lentiviral

Tags

HA and MYC

Expression

Mammalian

Mutation

C293S: mutation in the phosphatase domain

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
SBE-NLS-mCherry-P2A-CreERT2 PGK-rtTA3

Plasmid

#196936

Purpose

TGFbeta reporter driven nuclear mCherry and CreERT2 with PGK driven rtTA3

Depositor

Elaine Fuchs

Article

Oshimori et al Cell. 2015 Feb 26;160(5):963-976. doi: 1

Insert

mCherry

Use

Lentiviral and RNAi

Tags

P2A-CreERT2

Expression

Mammalian

Promoter

SBE

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pNIR-FbLAG30

Plasmid

#220740

Purpose

NIR-FbLAG30 expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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