162,981 results

1. tdTomato-Zyxin-6

   Plasmid

   #58148

   Purpose

   Localization: Focal Adhesions, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Article

   Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

   Insert

   Zyxin (ZYX Human)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

2. ATP1A1_G4_Q118R_Std_Dual_epegRNA_tevopreQ1

   Plasmid

   #187454

   Purpose

   Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA (ATP1A1 Human)
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-SauCas9

   Plasmid

   #174040

   Purpose

   In vitro transcription of SauCas9 mRNA

   Depositor

   James Gagnon

   Article

   Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

   Insert

   SauCas9
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSF-KpAGO207-1251

   Plasmid

   #117854

   Purpose

   Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

   Depositor

   Kotaro Nakanishi, Dinshaw Patel

   Article

   Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

   Insert

   Argonaute
   

   Use

   Tags

   6xHis-SUMO encoded in vector

   Expression

   Bacterial

   Mutation

   missing first 206 amino acid residues

   Promoter

   T7

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVA23-GG

   Plasmid

   #208013

   Purpose

   for cloning homology arms, with GFP as counter selection marker

   Depositor

   Pablo Ivan Nikel

   Article

   Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

   Insert

   msfGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   14G

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. human pirh2-(1-261)

   Plasmid

   #24878

   Depositor

   Cheryl Arrowsmith

   Article

   Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

   Insert

   Pirh2 (RCHY1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

7. PcDNA3.1-2Strep

   Plasmid

   #210343

   Purpose

   Empty Vector for molecular cloning

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   2Strep tag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUW-tetO-BCL6

   Plasmid

   #139816

   Purpose

   doxycycline-inducible expression of Human BCL6 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   BCL6 (BCL6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   T190A, E241G, L314M- please see depositor comments

   Promoter

   TRE-mCMV

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSP568

   Plasmid

   #139493

   Purpose

   Insertion of the pSTL1 promoter and 24 MS2 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pSTL1 24xMS2sl
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   pSTL1

   Available since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Crimson/P2A-ICP8 (pSLIK1)

    Plasmid

    #113856

    Purpose

    For Doxycycline-inducible expression of ICP8 synaptase, with red fluorescent protein gene E2-Crimson-P2A-ICP8

    Depositor

    Richard S Myers

    Article

    Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

    Insert

    ICP8
    

    Use

    Lentiviral

    Tags

    P2A/Crimson

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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