Skip to main content
Addgene
Showing: 1681 - 1720 of 5006 results

  1. pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)

    Plasmid
    #105922
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITR
    Depositor
    Marcella Patrick
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Tags
    Expression
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Available since
    Feb. 15, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    105922-plasmid-map-sequence-id-200599

    Enlarge

  5. pISV-CRISPR/Cas9PGmUbi

    Plasmid
    #209189
    Purpose
    Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection
    Depositor
    Christian Staehelin
    Inserts
    Cas9
    DsRed(deltaB)
    Use
    Tags
    NLS
    Expression
    Plant
    Mutation
    missing BsaI site and plant-codon optimized with …
    Promoter
    CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    209189-plasmid-map-sequence-id-419145

    Enlarge

  7. SpCas9 iFE

    Plasmid
    #190138
    Purpose
    Expresses SpCas9-POLB (iFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    SpCas9-POLB (HiFi iFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Promoter
    CBh
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190138-plasmid-map-sequence-id-374863

    Enlarge

  8. pGBW-m4133639

    Plasmid
    #152344
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152344-plasmid-map-sequence-id-300328

    Enlarge

  10. pIK86

    Plasmid
    #47933
    Purpose
    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.
    Depositor
    Iskra Katic
    Article
    Katic et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR and Unspecified
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47933-plasmid-map-sequence-id-68745

    Enlarge

  11. HPGD

    Plasmid
    #39154
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HPGD (HPGD Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pHN873

    Plasmid
    #178829
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Use
    Tags
    MBP, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178829-plasmid-map-sequence-id-351932

    Enlarge

  15. pKG113

    Plasmid
    #63073
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-T (CENPT Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    E. coli codon optimized. G217R mutation compared …
    Promoter
    Available since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63073-plasmid-map-sequence-id-134725

    Enlarge

  19. pGBW-m4133568

    Plasmid
    #152340
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152340-plasmid-map-sequence-id-420347

    Enlarge

  20. pGBW-m4133731

    Plasmid
    #152742
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152742-plasmid-map-sequence-id-313507

    Enlarge

  21. LZ3 iFE

    Plasmid
    #190143
    Purpose
    Expresses LZ3Cas9-POLB (LZ3 iFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    LZ3Cas9-POLB (LZ3_iFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190143-plasmid-map-sequence-id-374873

    Enlarge

  22. SpCas9 dFE

    Plasmid
    #190137
    Purpose
    Expresses SpCas9-T4pol (dFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    SpCas9-T4pol (dFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Promoter
    CBh
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190137-plasmid-map-sequence-id-382840

    Enlarge

  24. HSD11B1

    Plasmid
    #39155
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD11B1 (HSD11B1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    T220I; codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. pGBW-m4133256

    Plasmid
    #152133
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152133-plasmid-map-sequence-id-300772

    Enlarge

  26. pGBW-m4133586

    Plasmid
    #152328
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152328-plasmid-map-sequence-id-342371

    Enlarge

  27. pGBW-m4133862

    Plasmid
    #152487
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152487-plasmid-map-sequence-id-317734

    Enlarge

  28. pGBW-m4133872

    Plasmid
    #152464
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152464-plasmid-map-sequence-id-309465

    Enlarge

  29. pHH0103 KIBRA WW domain #2

    Plasmid
    #104230
    Purpose
    Bacterial expression of WW domain #2 from KIBRA
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    KIBRA WW domain #2 (WWC1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104230-plasmid-map-sequence-id-204290

    Enlarge

  30. pHH0103 YAP1 WW domain #2

    Plasmid
    #104228
    Purpose
    Bacterial expression of WW domain #2 from YAP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    YAP1 WW domain #2 (YAP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104228-plasmid-map-sequence-id-204296

    Enlarge

  31. pHH0103 YAP1 WW domain #1

    Plasmid
    #104227
    Purpose
    Bacterial expression of WW domain #1 from YAP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    YAP1 WW domain #1 (YAP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104227-plasmid-map-sequence-id-201508

    Enlarge

  32. GUCY1B3

    Plasmid
    #39035
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GUCY1B3 (GUCY1B1 Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. HSD17B14

    Plasmid
    #39160
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B14 (HSD17B14 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. pVG134

    Plasmid
    #227770
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Glu84Gly) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Glu84Gly mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227770-plasmid-map-sequence-id-458112

    Enlarge

  35. pVG178

    Plasmid
    #227761
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Glu84Lys) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Glu84KLys mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227761-plasmid-map-sequence-id-458111

    Enlarge

  36. pVG144

    Plasmid
    #227760
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Val121Met mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227760-plasmid-map-sequence-id-458109

    Enlarge

  37. pVG150

    Plasmid
    #227759
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Ser137Asn) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Ser137Asn mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227759-plasmid-map-sequence-id-458108

    Enlarge

  38. pVG124

    Plasmid
    #227758
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Thr83Ser mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227758-plasmid-map-sequence-id-458107

    Enlarge

  39. pVG121

    Plasmid
    #227757
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Gly82Val) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Gly80Glu mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227757-plasmid-map-sequence-id-458105

    Enlarge

  40. pVG123

    Plasmid
    #227755
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Ala157Val) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Ala157Val mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227755-plasmid-map-sequence-id-458101

    Enlarge

Showing: 1681 - 1720 of 5006 results