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pSbi-pur-EGFP-KSR1-CA3 (N-KSR)
Plasmid#217769PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseSleeping beauty transposase-compatible genome ins…TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400PromoterEF-1alpha promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
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FLAG-MARK3-CR
Plasmid#229422PurposeLentiviral or overpexression of cDNADepositorInsertMARK3-CR (MARK3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationsilent mutation CRISPR resistantPromoterEFSAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MARK2-CR
Plasmid#229421PurposeLentiviral or overpexression of cDNADepositorInsertMARK2-CR (MARK2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationsilent mutation CRISPR resistantPromoterEFSAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSbi-pur-KSR1-CA3-EGFP (C-KSR)
Plasmid#217768PurposeFluorescent reporter for ceramide (for stable mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseSleeping beauty transposase-compatible genome ins…TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterEF-1alpha promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
LV-AR-C619Y
Plasmid#171222PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: C619Y, mutation at the end of zinc finger 2.DepositorInsertAR-C619Y (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-C619YPromoterEF-1aAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-opCas12a-Ultra-6xNLS
Plasmid#209024PurposeLentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLSDepositorInsertopCas12a-Ultra-6xNLS-Flag-Myc-NeoR
UseCRISPR and LentiviralTagsFLAG and MYCExpressionMammalianMutationE174R/M537R/S542R/F870LPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast 3XFLAG-CHP1
Plasmid#173166PurposeRetroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1DepositorInsertCalcineurin Like EF-Hand Protein 1 (CHP1 Human)
UseRetroviralTags3XFLAGExpressionMutationSynonymous mutations at sgRNA sitesPromoterMMLVAvailable sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
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EFR D5_pECIA2
Plasmid#115119PurposeBait vector EFR D5_pECIA2 should be used with prey vector EFR D5_pECIA14.DepositorInsertAT5G20480 (EFR Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
EFR D5_pECIA14
Plasmid#114919PurposePrey vector EFR D5_pECIA14 should be used with bait vector EFR D5_pECIA2.DepositorInsertAT5G20480 (EFR Mustard Weed)
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
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HA-MST1-7A
Plasmid#229418PurposeLentiviral or overpexression of cDNADepositorInsertMST1-7A (MST1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (T175,T273,S320,S327,T340,T387…PromoterEFSAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
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sgMARK3-R-2
Plasmid#229438Purposeknockout of MARK3DepositorInsertsgMARK3-R-2 (MARK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
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HA-TAZ-4D
Plasmid#229416PurposeLentiviral or overpexression of cDNADepositorInsertTAZ-4D (WWTR1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S58,S60,S62,S90) mutated to DPromoterEFSAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MARK2-K82H-CR
Plasmid#229425PurposeLentiviral or overpexression of cDNADepositorInsertMARK2-K82H (MARK2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationKinase-dead mutant K82H; silent mutation CRISPR r…PromoterEFSAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MARK3-GK-CR
Plasmid#229424PurposeLentiviral or overpexression of cDNADepositorInsertMARK3-GK-CR (MARK3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationGatekeeper mutation M132G; silent mutation CRISPR…PromoterEFSAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MARK2-GK-CR
Plasmid#229423PurposeLentiviral or overpexression of cDNADepositorInsertMARK2-GK-CR (MARK2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationGatekeeper mutation M129G; silent mutation CRISPR…PromoterEFSAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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sgCDK1-ROSA
Plasmid#229443Purposeknockout of CDK1DepositorInsertsgCDK1-ROSA (CDK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-3-3
Plasmid#229442Purposeknockout of MARK2 and MARK3DepositorInsertsgMARK2-3-3 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-3-2
Plasmid#229441Purposeknockout of MARK2 and MARK3DepositorInsertsgMARK2-3-2 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-3-1
Plasmid#229440Purposeknockout of MARK2 and MARK3DepositorInsertsgMARK2-3-1 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK3-R-3
Plasmid#229439Purposeknockout of MARK3DepositorInsertsgMARK3-R-3 (MARK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK3-R-1
Plasmid#229437Purposeknockout of MARK3DepositorInsertsgMARK3-R-1 (MARK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-R-3
Plasmid#229436Purposeknockout of MARK2DepositorInsertsgMARK2-R-3 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-R-2
Plasmid#229435Purposeknockout of MARK2DepositorInsertsgMARK2-R-2 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-R-1
Plasmid#229434Purposeknockout of MARK2DepositorInsertsgMARK2-R-1 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-YAP-5D
Plasmid#229415PurposeLentiviral or overpexression of cDNADepositorInsertYAP-5D (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S128,S163,S350,T354,T398) mut…PromoterEFSAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-TAZ-4A
Plasmid#229414PurposeLentiviral or overpexression of cDNADepositorInsertTAZ-4A (WWTR1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S58,S60,S62,S90) mutated to APromoterEFSAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MAP4K4-10A
Plasmid#229403PurposeLentiviral or overpexression of cDNADepositorInsertMAP4K4 (MAP4K4 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S523,S536,S547,S643,T684,S726…PromoterEFSAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MAP4K4-10D
Plasmid#229404PurposeLentiviral or overpexression of cDNADepositorInsertMAP4K4 (MAP4K4 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S523,S536,S547,S643,T684,S726…PromoterEFSAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-YAP-5A
Plasmid#229413PurposeLentiviral or overpexression of cDNADepositorInsertYAP-5A (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S128,S163,S350,T354,T398) mut…PromoterEFSAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-SpCas9D10A nickase
Plasmid#216736PurposeExpresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRTagsExpressionMammalianMutationD10APromoterEF-1-alpha coreAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Tafazzin [N173B/13R]
Plasmid#206539PurposeMammalian Expression Plasmid of anti-Tafazzin (Human). Derived from hybridoma N173B/13.DepositorInsertanti-Tafazzin (Homo sapiens) recombinant Mouse monoclonal antibody (TAFAZZIN Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pQE-WT-T3-R5-RV
Plasmid#63628PurposeT3-R5-RV RNAP mutantDepositorInsertT3-R5-RV RNAP
UseTagsExpressionMutationT745K, N748D, L749M, M750I, H772R, E775VPromoterT5/LacAvailable sinceApril 15, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits
