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Plasmid#209304PurposeDoxycycline-gated expression plasmid (TetOFF) for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in miceDepositorInsertGEM7
UseAAVTagsHaloTagExpressionMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Homology Arms-BRD4-HaloTag
Plasmid#228575PurposeCRISPR donor plasmid to tag human BRD4 with Halo tag; for CRISPR-mediated gene insertionDepositorInsertHomology Arms-BRD4-HaloTag (BRD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-BRD4-HaloTag-Guide-hspCas9
Plasmid#228576PurposeExpression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertionDepositorInsertBRD4 gRNA (BRD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IBU-pSu9-HaloTag7-SNAP
Plasmid#217319PurposeExpresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.DepositorInsertNeurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
UseRetroviralTagsHaloTag and SNAPExpressionMutationPromoterCMVAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-PILATeS-RGD (200nM)
Plasmid#216716PurposeExpresses HaloTag-PILATeS-RGD with 200 nM BiT tag in bacteriaDepositorInsertHaloTag-PILATeS-RGD
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-PILATeS-RGD (700pM)
Plasmid#216714PurposeExpresses HaloTag-PILATeS-RGD with 700 pM BiT tag in bacteriaDepositorInsertHaloTag-PILATeS-RGD
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-MDC1 BRCT domain deletion
Plasmid#207110PurposepHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the BRCT domain in human cells.DepositorInsertHaloTag-MDC1 BRCT domain deletion
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP
Plasmid#179717PurposeLentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFPDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-Halo-CD4-bla
Plasmid#189982PurposeDonor Vector to integrate HaloTag C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertHaloTag
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hCRY2-Halo-CD4-bla
Plasmid#189986PurposeDonor Vector to integrate HaloTag C-terminal into the human CRY2 Locus to express it as a fusion proteinDepositorInsertHaloTag
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-XPACK-DNAJC5 (L115R)- HaloTag
Plasmid#205712PurposeMammalian expression of XPACK-DNAJC5 (L115R)-HaloTagDepositorInsertDNAJC5 (DNAJC5 Human)
UseTagsHaloTag and XPACK (GCINSKRKD)ExpressionMammalianMutationL115RPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-V5-HisB-ObKHC-K282R-HaloTag
Plasmid#201552PurposeExpresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cellsDepositorInsertKinesin-1
UseTagsHaloTagExpressionInsectMutationchanged lysine 282 to arginine (numbering based o…PromoterAc5Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.HALO-KIF3A tail 419-702
Plasmid#193924PurposeMammalian expression of Halo-tagged Kif 3A tail 419-702DepositorInsertKif 3A (KIF3A Human)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C ACT-DCC-Halo
Plasmid#195148Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
UseTagsExpressionMammalianMutationmutations to make si RNA resistant and remove act…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BRAF-REG-T241P-Halo
Plasmid#202549PurposeMammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag. The REG domain contains the RASopathy mutation, T241P, within its cysteine-rich domain.DepositorInsertBRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
UseTagsHaloTagExpressionMammalianMutationThreonine 241 mutated to prolinePromoterCMVAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-Halotag-RfA
Plasmid#186377PurposeDestination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3B tail 391-747
Plasmid#193929PurposeMammalian expression of Halo-tagged Kif 3B tail 391-747DepositorInsertKif 3B (Kif3b Mouse)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP
Plasmid#185679PurposeLow-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.DepositorInsertERmembrane-2xHaloTag-KKMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-H189AD194A-Halo
Plasmid#195168Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tagDepositorInsertABHD16A (ABHD16A Human)
UseTagsHalo tagExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-S355A-Halo
Plasmid#195169Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tagDepositorInsertABHD16A (ABHD16A Human)
UseTagsHalo tagExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only