We narrowed to 9,644 results for: SUB

1. nonphosphorylatable Cenp-B-targted Aurora B FRET sensor

   Plasmid

   #45230

   Depositor

   Michael Lampson

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   nonphos Cenp-B targeted Aurora B FRET sensor
   

   Expression

   Mammalian

   Mutation

   t-a mutation in substrate sequence

   Available Since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Myc-gamma2s-EGFP

   Plasmid

   #118040

   Purpose

   GABAA receptor expression (gamma2 short subunit) double tagged, near N terminus and at C-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG2 (Gabrg2 Rat)
   

   Tags

   EGFP and cMyc epitope EQKLISEEDL inserted between…

   Expression

   Mammalian

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE puro rat IRS-3

   Plasmid

   #11362

   Depositor

   Ronald Kahn

   Article

   Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

   Insert

   IRS-3 (Irs3 Rat)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pGADT7-AP-4 β4

   Plasmid

   #198327

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

   Insert

   AP-4 β4
   

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Contains L480S substitution

   Promoter

   ADH1

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGADT7-AP-1 σ1C

   Plasmid

   #198336

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

   Insert

   AP-1 σ1C
   

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   S148C substitution

   Promoter

   ADH1

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Myc-gamma1 (in pCAGGS)

   Plasmid

   #118039

   Purpose

   GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG1 (Gabrg1 Rat)
   

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. MPS-3

   Plasmid

   #113071

   Purpose

   Expresses worm MPS-3 in mammalian cells

   Depositor

   Federico Sesti

   Article

   Park et al J Biol Chem. 2005 Jun 10;280(23):21893-9

   Insert

   MiRP K+ channel accessory Subunit 3 (mps-3 Nematode)
   

   Expression

   Mammalian

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOX + JShal XR

   Plasmid

   #16148

   Depositor

   Larry Salkoff

   Article

   Jegla et al J Neurosci. 1997 Jan 1. 17(1):32-44.

   Insert

   Polyorchis penicillatus potassium channel gamma subunit (LOC721209, Ggamma1 jelly fish)
   

   Use

   Xenopus oocyte expression

   Mutation

   N/A

   Available Since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

9. BS.YxiN.Twin1.a1-368.wt

   Plasmid

   #13528

   Depositor

   David McKay

   Article

   Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

   Insert

   B. subtilis DEAD-box helicase YxiN first two domains
   

   Tags

   intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   deleted amino acid residues 369-479

   Available Since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. BS.YxiN.Twin1.a404-479.wt

    Plasmid

    #13529

    Depositor

    David McKay

    Article

    Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

    Insert

    B. subtilis DEAD-box helicase YxiN specific RNA binding domain
    

    Tags

    intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    delete amino acid residues 1-403

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. Red Glifon 300

    Plasmid

    #163115

    Purpose

    Red fluorescent protein-based glucose sensor for mammalian cells with higer affinity

    Depositor

    Takashi Tsuboi

    Article

    Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

    Insert

    Red Glifon 300
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Red Glifon 3000

    Plasmid

    #163116

    Purpose

    Red fluorescent protein-based glucose sensor for mammalian cells with lower affinity

    Depositor

    Takashi Tsuboi

    Article

    Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

    Insert

    Red Glifon 3000
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Red cGull

    Plasmid

    #191853

    Purpose

    Expresses biosensor for cGMP in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

    Insert

    Red cGull
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-NtLNtC60αC20C60β

    Plasmid

    #160874

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Expression

    Bacterial

    Promoter

    rbcL: pBAD, chaperones: T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDF-NtXNtS1R1R2B2

    Plasmid

    #160882

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcS-S1, rbcX, raf1, raf2, bsd2
    

    Expression

    Bacterial

    Promoter

    rbcS-S1: T7, chaperones: T7

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Kras-Src FRET biosensor

    Plasmid

    #78302

    Purpose

    FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

    Insert

    Src biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAR357_SFP_pCDF-1b

    Plasmid

    #122851

    Purpose

    Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    Sfp
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-MCN-10His-mStayGold (E138D)

    Plasmid

    #211361

    Purpose

    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    E138D

    Promoter

    T7

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. μ2-HA-WT

    Plasmid

    #32752

    Depositor

    Alexander Sorkin

    Article

    Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

    Insert

    μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. qi5

    Plasmid

    #24501

    Depositor

    Bruce Conklin

    Article

    Conklin et al Nature. 1993 May 20. 363(6426):274-6.

    Insert

    Gq alpha (Gnaq Mouse)
    

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    Gq alpha with the C-terminal amino acids changed …

    Available Since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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