We narrowed to 18,336 results for: met

1. Twitch-3 pcDNA3

   Plasmid

   #49532

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-3
   

   Use

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-Ef1a-DIO_EGFP-RandE3

   Plasmid

   #79872

   Purpose

   For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. STARR-seq luciferase validation vector_SCP1_empty

   Plasmid

   #99299

   Purpose

   Luciferase validation vector; empty vector without insert to determine baseline

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GA-DEVD-GB-NES

   Plasmid

   #61020

   Purpose

   green ddFP based caspase-3 biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP A and ddGFP B
   

   Use

   Tags

   HindIII and NES sequence LALKLAGLDIGS placed afte…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pPPC001

   Plasmid

   #171138

   Purpose

   For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   Sp.pCas9, BBa_J23107

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Twitch-3 pRSETB

   Plasmid

   #48204

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-3A
   

   Use

   Tags

   6xHIS, cpVenusCD, and mCerulean3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pUASp-FMW-attB-AthHen1

   Plasmid

   #104957

   Purpose

   Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila germline tissues using Gal4 drivers

   Depositor

   Stefan Ameres

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Insert

   Hen1 (HEN1 Mustard Weed)
   

   Use

   Tags

   FLAG Myc

   Expression

   Insect

   Mutation

   Promoter

   pTrasposase promoter

   Available Since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

   Plasmid

   #172317

   Purpose

   CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUASt-FMW-attB-AthHen1

   Plasmid

   #104958

   Purpose

   Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila using somatic tissue Gal4 drivers

   Depositor

   Stefan Ameres

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Insert

   Hen1 (HEN1 Mustard Weed)
   

   Use

   Tags

   FLAG Myc

   Expression

   Insect

   Mutation

   Promoter

   Hsp70 promoter

   Available Since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET30a-GABAT

    Plasmid

    #239572

    Purpose

    Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

    Depositor

    William Plaxton

    Article

    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

    Insert

    4-aminobutyrate aminotransferase (gabT E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET30a-SSDH

    Plasmid

    #239573

    Purpose

    Expresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

    Depositor

    William Plaxton

    Article

    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

    Insert

    Succinate-semialdehyde dehydrogenase (gabD E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKG-GW2

    Plasmid

    #203164

    Purpose

    Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using methionine selection

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMVQA

    Plasmid

    #197894

    Purpose

    pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

    Depositor

    Thomas Klimkait

    Article

    Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

    Insert

    HIV-1 3' end (HXB2: 9045 till poly-A(10)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pM-mRuby2

    Plasmid

    #176550

    Purpose

    Expression of mRuby2 for auxotrophic selection in the absence of methionine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    yomRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pM-eCFP

    Plasmid

    #176558

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of methionine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    eCFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Twitch-2C pRSETB

    Plasmid

    #104500

    Purpose

    fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-2C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

17. FH-TET1-pEF

    Plasmid

    #49792

    Purpose

    This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

    Depositor

    Anjana Rao

    Article

    Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

    Insert

    Tet methylcytosine dioxygenase 1 (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJBEI-6409

    Plasmid

    #47048

    Purpose

    BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    

    Use

    Synthetic Biology; Bglbrick

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacUV5

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. STARR-seq luciferase validation vector_ORI_neg.cont

    Plasmid

    #99315

    Purpose

    Negative control for luciferase validation vectors

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    neutral region; chr3: 55198740-55200041
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. single polypeptide FPX biosensor for calcium ion

    Plasmid

    #60887

    Purpose

    FPX biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for calcium ion
    

    Use

    Tags

    HindIII site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge