174,739 results

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mCherry-CRY2-iSH2

Plasmid

#66839

Purpose

Expression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

iSH2

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

Plasmid

#64113

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP rbFOX1

Plasmid

#63085

Purpose

Mammalian expression of EGFP tagged FOX1

Depositor

Nicolas Charlet-Berguerand

Insert

RBFOX1 (RBFOX1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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YFP-Parkin-IRES-zeo

Plasmid

#61728

Purpose

Expression of human Parkin fused to eYFP

Depositor

Douglas Green, Stephen Tait

Article

Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

Insert

Parkin (PRKN Human)

Tags

eYFP

Expression

Mammalian

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1512

Plasmid

#62366

Purpose

N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pTRE2

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-UbC

Plasmid

#62806

Purpose

To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

Depositor

Jae Lee, Pantelis Tsoulfas

Article

Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

Type

Empty backbone

Use

AAV; Eucaryotic

Expression

Mammalian

Promoter

hUbC

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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eGFPbait-E2A-KalTA4-pA donor vector

Plasmid

#61069

Purpose

for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)

Depositor

Filippo Del Bene

Article

Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

Inserts

eGFPbait
KalTA4

Use

Zebrafish expression

Tags

E2A

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pSBbi-RH

Plasmid

#60516

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and hygromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pBudORF1-CH

Plasmid

#51290

Purpose

expresses the codon optimized ORF1 of L1RP with his tag

Depositor

Astrid Roy-Engel

Article

Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

Insert

L1 ORF1 (L1RE1 Human)

Tags

HIS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-loxP-hKLF2

Plasmid

#60850

Purpose

Lentiviral DOX-inducible expression vector for human KLF2

Depositor

Rudolf Jaenisch

Article

Theunissen et al Cell Stem Cell. 2014 Oct 2;15(4):471-87.

Insert

KLF2 (KLF2 Human)

Use

Lentiviral

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG_mRuby2_smFP OLLAS

Plasmid

#59761

Purpose

encodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

mRuby2_smFP_OLLAS

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-Intersectin Long

Plasmid

#47395

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

intersectin long (ITSN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCS-H2B-cerulean

Plasmid

#53748

Purpose

Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

Cerulean

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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mTFP1-C1

Plasmid

#54613

Purpose

Localization: C1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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mKO2-C1

Plasmid

#54494

Purpose

Localization: C1 Cloning Vector, Excitation: 551, Emission: 565

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

Type

Empty backbone

Tags

mKO2

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pNCS-mCardinal2

Plasmid

#52631

Purpose

bacterial expression vector with far-red FP mCardinal2

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mCardinal2

Expression

Bacterial

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only

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MR015-H11-L2-TALEN

Plasmid

#51554

Purpose

Expresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.

Depositor

Michele Calos

Article

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

H11-L2 TALEN

Use

TALEN

Expression

Mammalian

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry Vinculin

Plasmid

#50527

Depositor

Kenneth Yamada

Insert

Vinculin (VCL Chicken)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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CAG-Flex-TC66T

Plasmid

#48331

Purpose

Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVA

Depositor

Liqun Luo

Article

Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

Insert

TVA receptor mCherry fusion with the 66T mutation

Use

AAV and Cre/Lox

Tags

mCherry fusion to TVA receptor

Expression

Mammalian

Mutation

66T

Promoter

CAG

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pTALYM4SpMi-05

Plasmid

#47880

Purpose

TALE-mRuby2 against Mus spretus/musculus minor satellite sequence

Depositor

Maria-Elena Torres-Padilla

Article

Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

Insert

TALE-mRuby2

Use

TALEN

Tags

mRuby2

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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