We narrowed to 11,803 results for: nsf

1. pAAV-CAG-DIO(ChRger1-TS-YFP)

   Plasmid

   #127245

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger1-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-DIO(ChRger3-TS-YFP)

   Plasmid

   #127242

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger3-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ETRAMP10.2-bio

   Plasmid

   #47789

   Purpose

   Expresses enzymatically monobiotinylated full-length ETRAMP10.2 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised ETRAMP10.2
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. RAP3-bio

   Plasmid

   #47796

   Purpose

   Expresses enzymatically monobiotinylated full-length RAP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised RAP3
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. SIN-cPPT-H1-sgHTT1-PGK-mCherry-WPRE

   Plasmid

   #87885

   Purpose

   To produce lentiviral vector for HTT editing

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   shHTT1, cherry
   

   Use

   Lentiviral

   Promoter

   H1, PGK

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. DCT.VV.Orf35

   Plasmid

   #12723

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf35 (J3R Vaccinia Virus)
   

   Expression

   Mammalian

   Available Since

   Dec. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pQC V5 HuR.wt IRES Puro

   Plasmid

   #110386

   Purpose

   γ-Retroviral transfer vector for expressing HuR (wild type), IRES-driven Puromycin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Retroviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC1-pCR8-dCas9VP48

   Plasmid

   #48214

   Purpose

   dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP48 activation domain
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag and VP48

   Mutation

   D10A;H840A

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. P38-bio

   Plasmid

   #47727

   Purpose

   Expresses enzymatically monobiotinylated full-length P38 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised P38
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCA-G-intron(Neo)-T(LNL)

    Plasmid

    #36907

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    Neo
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDSM-ab-Psptef1-NAT-Ttip1

    Plasmid

    #127747

    Purpose

    Yeast pathway position 2. NatMX transcription unit with the S. paradoxus TEF1 promoter and TIP1 terminator. Nourseothricin resistance.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    nourseothricin N-acetyltransferase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Psptef1

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMflPT-o4

    Plasmid

    #101315

    Purpose

    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Inserts

    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    pac resistance gene
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMflST-o4

    Plasmid

    #101316

    Purpose

    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Inserts

    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    aadA1 resistance gene
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnC

    Plasmid

    #114374

    Purpose

    expresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-TlcnC (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and TlcnC

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSRP1-bio

    Plasmid

    #47736

    Purpose

    Expresses enzymatically monobiotinylated full-length MSRP1 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSRP1
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MSRP2-bio

    Plasmid

    #47737

    Purpose

    Expresses enzymatically monobiotinylated full-length MSRP2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSRP2
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSRP3-bio

    Plasmid

    #47738

    Purpose

    Expresses enzymatically monobiotinylated full-length MSRP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSRP3
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Pf34-bio

    Plasmid

    #47784

    Purpose

    Expresses enzymatically monobiotinylated full-length Pf34 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised Pf34
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenCMV-PMMars1CyAVPR2

    Plasmid

    #75226

    Purpose

    Lentiviral transfer vector encoding Mars1Cy-tagged AVPR2

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    Mars1Cy-AVPR2 (AVPR2 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA epitope

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUASp-Flag-Myc-Qin-E3-attB

    Plasmid

    #44209

    Depositor

    Phillip Zamore

    Article

    Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

    Insert

    Qin (qin Fly)
    

    Use

    Fly transformation

    Tags

    3xFlag and 6xMyc

    Mutation

    lacking 5xTudor domains on C-term; aa1-274 only

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only